Bacterial artificial chromosome

From WikiMD's medical encyclopedia

A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells such as E. coli.


Use

BACs are often used in connection with DNA sequencing.

Segment length

  • Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.
  • The BACs, with their inserted DNA, are then taken up by bacterial cells.
  • As the bacterial cells grow and divide, they amplify the BAC DNA, which can then be isolated and used in sequencing DNA.

Frequently asked questions

Chromosome-DNA-gene copy
Chromosome-DNA-gene copy

Help answer these questions.

  • How do bacterial artificial chromosomes work?
  • How do you make a bacterial artificial chromosome?
  • How does a bacterial artificial chromosome BAC differ from a plasmid?
  • What must be present in artificial chromosome?
  • What are artificial chromosomes used for?
  • What is YAC and BAC?
  • What are BAC transgenic mice?
  • What is the F factor in bacteria?
  • Is YAC a plasmid?
  • What is BAC system?
  • What is an advantage of BACs and YACs over plasmids?
  • What is a BAC vector?
  • Who is YAC?
  • Is YAC a vector?
  • Is YAC circular or linear?
  • What are BACs and YACs How are they different?
  • What is the difference between plasmid and cosmid?
  • What do the sequence Tel signify in a YAC vector?
  • What is the cen4 region in the YAC vector?
  • What are cosmid vectors?
  • How can the presence of insert DNA in the YAC vector be checked?

External links

Wikipedia

Latest research

PubMed


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