Immunolabeling

Immunolabeling (pronunciation: /ˌɪm.jʊ.noʊˈleɪ.bəl.ɪŋ/) is a technique used in microscopy to detect a specific protein or antigen in cells or tissues.

Etymology

The term "immunolabeling" is derived from the words "immune", referring to the immune system's response to antigens, and "labeling", which refers to the marking of these antigens for identification.

Technique

Immunolabeling involves the use of antibodies that are directly or indirectly labeled with a detectable marker, such as a fluorescent dye or an enzyme. These antibodies bind specifically to the protein or antigen of interest, allowing it to be visualized under a microscope.

There are two main types of immunolabeling: direct and indirect. In direct immunolabeling, the primary antibody is directly conjugated to the marker. In indirect immunolabeling, a secondary antibody, which is conjugated to the marker, is used to bind to the primary antibody.

Applications

Immunolabeling is widely used in biological and medical research to study the location and function of proteins in cells and tissues. It is also used in diagnostic pathology to detect the presence of specific antigens in tissue samples, which can help in the diagnosis of diseases such as cancer.

Related Terms

• Immunohistochemistry
• Immunofluorescence
• Antibody
• Antigen
• Microscopy

External links

• Medical encyclopedia article on Immunolabeling
• Wikipedia's article - Immunolabeling

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