PFA-100

PFA-100 is a diagnostic instrument used in the field of hematology to measure platelet function. The PFA-100 system is designed to simulate the process of primary hemostasis and thus, it measures the time taken for a platelet plug to form, which is referred to as the closure time. This system is often used to detect platelet function defects, especially in conditions such as Von Willebrand disease and platelet function disorders.

Overview

The PFA-100 system works by aspirating citrated whole blood from a test cartridge through a capillary and then an aperture cut into a membrane coated with either collagen and epinephrine or collagen and ADP. This process is carried out under conditions of high shear stress. The platelets adhere to the collagen, become activated, and then aggregate to form a platelet plug that occludes the aperture.

Clinical Significance

The PFA-100 system is used in the diagnosis and monitoring of various hematological disorders. It is particularly useful in the diagnosis of Von Willebrand disease, a common inherited bleeding disorder. The system can also be used to monitor the efficacy of treatment in patients with this disease.

In addition, the PFA-100 system can be used to detect platelet function defects in patients with uremia, myeloproliferative disorders, and those undergoing cardiopulmonary bypass surgery. It can also be used to assess the effect of antiplatelet therapy in patients with coronary artery disease.

Limitations

While the PFA-100 system is a valuable tool in the assessment of platelet function, it has some limitations. For instance, it may not detect mild platelet function defects. Furthermore, the results can be influenced by various factors such as hematocrit, red cell indices, and the presence of certain medications.

See Also

• Hematology
• Platelet function disorders
• Von Willebrand disease
• Platelet

References

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