Gene targeting

Gene Splicing is a molecular biology technique that manipulates the genetic material of an organism, specifically its DNA. This technique is used to remove, add or alter genetic material, or 'genes', in a DNA molecule.

Overview

Gene splicing involves the cutting and pasting of gene sequences to alter the genetic material of an organism. This is achieved through the use of specialized enzymes known as restriction enzymes that can cut DNA at specific sequences. Once the DNA is cut, new genetic material can be inserted at the cut site. This new genetic material can be from the same organism, a different organism, or it can be synthetic.

Process

The process of gene splicing begins with the isolation of the gene of interest. This gene is then inserted into a vector, which is a DNA molecule that can replicate within a living cell. The vector is then introduced into a host organism, where it replicates and produces many copies of the inserted gene. This process is known as cloning.

Once the gene has been cloned, it can be manipulated further. For example, specific parts of the gene can be removed or altered, or new genetic material can be inserted. This is achieved through the use of restriction enzymes, which cut the DNA at specific sequences, and ligase enzymes, which join DNA fragments together.

Applications

Gene splicing has a wide range of applications in various fields such as medicine, agriculture, and biotechnology. In medicine, it is used in the production of recombinant proteins such as insulin and growth hormones. In agriculture, it is used to create genetically modified organisms (GMOs) with desirable traits such as resistance to pests or improved nutritional content. In biotechnology, it is used in the production of biofuels and bioplastics.

Ethical Considerations

While gene splicing has many potential benefits, it also raises a number of ethical issues. These include concerns about the potential risks of genetically modified organisms (GMOs), the patenting of life forms, and the potential for misuse of the technology.

See Also

• Genetic Engineering
• Recombinant DNA
• Genetically Modified Organisms

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  Chimeric mouse with pups

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  Physcomitrella knockout mutants

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  Genetic engineering subtypes

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  Gene targeting mechanism vs non-homologous end joining