Transfection
Transfection
Transfection (/trænsˈfɛkʃən/) is a process in which nucleic acids such as DNA or RNA are introduced into cells. This process is used in cell biology to study and modify gene expression and protein function.
Etymology
The term "transfection" is a portmanteau of "trans-" and "infection". "Trans-" is a prefix from Latin meaning "across", "beyond" or "through", and "-fection" is derived from the Latin word 'infectio', meaning 'to infect'. The term was coined in the mid-20th century.
Process
Transfection can be performed using various methods, including calcium phosphate, electroporation, microinjection, and liposomes. The choice of method depends on the type of cells being transfected, the condition of the cells, and the downstream applications.
Applications
Transfection is used in various fields of study, including genetics, cell biology, and molecular biology. It is used to study gene function and regulation, protein expression, and cell growth and differentiation. It is also used in the production of recombinant proteins and vaccines, and in gene therapy.
Related Terms
- Gene expression
- Protein function
- Cell biology
- Molecular biology
- Recombinant proteins
- Gene therapy
- Vaccines
External links
- Medical encyclopedia article on Transfection
- Wikipedia's article - Transfection
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