Cohn process

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Cohn Process

The Cohn Process (pronounced: kohn proh-sess) is a method used in the fractionation of plasma, a component of blood, to obtain various proteins such as albumin, immunoglobulins, and fibrinogen. This process was developed by Dr. Edwin J. Cohn, a biochemist at Harvard Medical School, during the 1940s.

Etymology

The term "Cohn Process" is named after its developer, Dr. Edwin J. Cohn. The word "process" is derived from the Latin processus, meaning "progression, course."

Description

The Cohn Process involves a series of physical and chemical procedures to separate different proteins from plasma. The process begins with the collection of plasma from blood. The plasma is then subjected to cold ethanol precipitation under varying conditions of temperature, ethanol concentration, pH, and ionic strength. This results in the separation of plasma into five fractions, each containing different proteins.

Related Terms

  • Plasma Fractionation: The general process of separating the various components of blood plasma.
  • Blood Plasma: The liquid component of blood, in which the blood cells are suspended.
  • Protein: A complex molecule composed of amino acids that performs a vast array of functions within organisms.
  • Albumin: A type of protein that is soluble in water and coagulable by heat, such as that found in egg white, blood serum, and many other animal and plant tissues and fluids.
  • Immunoglobulins: Any of a class of proteins present in the serum and cells of the immune system, that function as antibodies.
  • Fibrinogen: A soluble protein present in blood plasma, from which fibrin is produced by the action of the enzyme thrombin.

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