Coliphage

Coliphage

A coliphage is a type of bacteriophage that specifically infects Escherichia coli (E. coli) bacteria. Bacteriophages, often referred to simply as phages, are viruses that infect and replicate within bacteria. Coliphages are used extensively in molecular biology and genetic engineering due to their specificity and ability to transfer genetic material.

Types of Coliphages

Coliphages can be broadly classified into two categories based on their life cycles: lytic and lysogenic.

• Lytic Coliphages: These phages infect the E. coli cell, replicate rapidly, and cause the cell to lyse (burst), releasing new phage particles. An example of a lytic coliphage is T4 phage.
• Lysogenic Coliphages: These phages integrate their genetic material into the host cell's genome and replicate along with it without causing immediate lysis. An example of a lysogenic coliphage is Lambda phage.

Applications

Coliphages have several important applications in science and industry:

• Molecular Biology: Coliphages are used as vectors for cloning and manipulating genes. The Lambda phage is a well-known vector used in recombinant DNA technology.
• Water Quality Testing: Coliphages are used as indicators of fecal contamination in water. Their presence suggests the potential presence of pathogenic E. coli strains.
• Phage Therapy: There is growing interest in using coliphages to treat bacterial infections, especially those caused by antibiotic-resistant strains of E. coli.

Life Cycle

The life cycle of a coliphage involves several stages:

1. Attachment: The phage attaches to specific receptors on the surface of the E. coli cell. 2. Penetration: The phage injects its genetic material into the host cell. 3. Replication: The phage's genetic material takes over the host's cellular machinery to replicate its own DNA and produce phage proteins. 4. Assembly: New phage particles are assembled within the host cell. 5. Release: The host cell lyses, releasing new phage particles to infect other E. coli cells.

Related Pages

• Bacteriophage
• Escherichia coli
• Lytic cycle
• Lysogenic cycle
• Lambda phage
• T4 phage
• Recombinant DNA
• Phage therapy

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