Eastern blot
Eastern blot
The Eastern blot is a biochemical technique used in molecular biology to detect specific protein molecules from among a mixture of proteins. This method was developed after the Western blot, Southern blot, and Northern blot techniques, hence the name "Eastern blot".
Pronunciation
- IPA: /ˈiːstərn blɒt/
Etymology
The term "Eastern blot" was coined in reference to the directional naming convention of other blotting methods. The Western blot was named as such because it was developed after the Southern blot, and the Northern blot was named in a similar fashion. The "Eastern" in "Eastern blot" continues this trend.
Method
The Eastern blot method involves transferring proteins from a gel onto a membrane, followed by detection using antibodies. The proteins can be post-translationally modified, and these modifications can be detected by the Eastern blot. This technique is particularly useful in the study of protein modifications and protein-protein interactions.
Related Terms
External links
- Medical encyclopedia article on Eastern blot
- Wikipedia's article - Eastern blot
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