Dot blot
Dot blot
Dot blot (pronunciation: /dɒt blɒt/) is a technique in Molecular Biology used to detect, analyze, and identify proteins, biomolecules, and DNA sequences. It is a simplified version of the Western blot method, and it is named for the dot-shaped results it produces.
Etymology
The term "Dot blot" is derived from the English words "dot," referring to the small, circular marks made on the membrane, and "blot," which is a method of transferring proteins, DNA, and RNA onto a carrier (like a nitrocellulose or PVDF membrane) so they can be detected and analyzed.
Procedure
In a Dot blot procedure, samples are not separated by electrophoresis before blotting. Instead, the samples are directly blotted onto the membrane. This method is particularly useful when the researcher knows the exact sequence or protein they are studying and does not require the separation provided by electrophoresis.
Applications
Dot blot is widely used in various fields of study, including genetics, oncology, and immunology. It is particularly useful in detecting specific DNA or RNA sequences in a mixture and in identifying specific proteins in a complex mixture.
Related Terms
- Western blot: A technique used to separate and identify proteins.
- Southern blot: A method used to check for the presence of a specific DNA sequence in a DNA sample.
- Northern blot: A technique used to study gene expression by detection of RNA (or isolated mRNA) in a sample.
External links
- Medical encyclopedia article on Dot blot
- Wikipedia's article - Dot blot
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