Viral transport medium
Viral Transport Medium (VTM) is a type of cell culture medium used to preserve the viability of viruses during transport from the collection site to the laboratory for further testing. It is a crucial component in the process of virus detection and diagnosis.
Composition
The composition of Viral Transport Medium can vary, but it typically contains a balanced salt solution, such as phosphate-buffered saline (PBS), and additional protective components. These may include protein sources like gelatin or serum albumin, and antibiotics to inhibit bacterial and fungal contamination. Some formulations may also contain a pH indicator or a cryoprotectant like glycerol.
Usage
Viral Transport Medium is used in the collection, transport, maintenance and long term freeze storage of viruses. It is capable of maintaining the optimal viability and virulence of the viral sample. The medium is used in conjunction with a swab, typically a nasopharyngeal swab or oropharyngeal swab, to collect the sample from the patient. The swab is then placed into a vial containing the medium.
Importance
The use of Viral Transport Medium is critical in virology, epidemiology, and public health. It allows for accurate testing and diagnosis of viral infections, including influenza, herpes simplex virus, varicella-zoster virus, and SARS-CoV-2, the virus responsible for COVID-19. The medium preserves the integrity of the virus, allowing for accurate PCR testing and viral culture.
See Also
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Contributors: Prab R. Tumpati, MD