PulseNet

PulseNet is a national laboratory network that connects foodborne illness cases to detect outbreaks. The network is coordinated by the Centers for Disease Control and Prevention (CDC) and includes public health laboratories in all 50 states in the United States. PulseNet uses DNA fingerprinting, or patterns of DNA, of bacteria making people sick to link cases that may be part of a larger, possibly multi-state outbreak, leading to a faster outbreak response.

History

PulseNet was established in 1996 by the CDC in response to a large outbreak of E. coli O157:H7 infections in the western United States. The network was created to improve the detection and response to foodborne disease outbreaks.

Methodology

PulseNet uses Whole Genome Sequencing (WGS) to identify the DNA fingerprint of the bacteria causing illness. This information is then used to link cases of illness that may be part of a larger outbreak. The network also uses Pulsed-field Gel Electrophoresis (PFGE) and other advanced molecular typing methods.

Role in Outbreak Detection

PulseNet plays a critical role in detecting foodborne illness outbreaks. The network allows public health officials to identify outbreaks early, often before they are large enough to be detected through traditional methods. This early detection allows for a faster response, which can reduce the number of illnesses and prevent further spread of the disease.

See Also

• Foodborne illness
• Centers for Disease Control and Prevention
• DNA fingerprinting
• Whole Genome Sequencing
• Pulsed-field Gel Electrophoresis