O-Dianisidine
O-Dianisidine
O-Dianisidine is an organic compound with the formula (CH_O)_C_H_NH_. It is a derivative of benzidine and is used in various applications, particularly in the field of biochemistry and histology.
Chemical Properties
O-Dianisidine is a diamine, meaning it contains two amine groups. The presence of methoxy groups on the benzene ring makes it a derivative of anisidine. It is typically found as a white to light brown crystalline powder.
Uses
O-Dianisidine is primarily used as a chromogenic substrate in enzyme assays. It is particularly useful in the detection of peroxidase activity. When oxidized by peroxidase in the presence of hydrogen peroxide, O-Dianisidine forms a colored product, which can be measured spectrophotometrically.
In histology, O-Dianisidine is used as a staining agent. It is employed in the detection of hemoglobin and other heme-containing compounds in tissue sections.
Safety
O-Dianisidine is considered a potential carcinogen and should be handled with care. Proper laboratory safety protocols should be followed when working with this compound, including the use of personal protective equipment (PPE) such as gloves and goggles.
Related Compounds
O-Dianisidine is related to other aromatic amines such as benzidine and o-tolidine. These compounds share similar chemical structures and are used in similar applications, particularly in the field of dye and pigment production.
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