Bacterial culture

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Bacterial Culture

Bacterial culture (pronunciation: /bækˈtɪərɪəl ˈkʌltʃər/) is a method used in microbiology to multiply bacteria by letting them reproduce in predetermined culture media under controlled laboratory conditions.

Etymology

The term "bacterial culture" is derived from the Latin bacterium meaning "small staff" and the Latin cultura meaning "growing, cultivation".

Procedure

The process of bacterial culture involves the following steps:

  1. Inoculation: The bacteria to be cultured are introduced into a culture medium. This is done in a sterile environment to prevent contamination.
  2. Incubation: The inoculated media is kept in an incubator at a specific temperature to allow the growth of bacteria.
  3. Isolation: After incubation, the bacteria colonies are isolated for further study.
  4. Identification: The isolated bacteria are identified by their biochemical characteristics and other testing methods.

Related Terms

  • Agar plate: A Petri dish filled with agar as a growth medium used for culturing microorganisms.
  • Colony: A visible mass of microorganisms all originating from a single mother cell.
  • Streaking: A method in microbiology used to isolate a pure strain from a single species of microorganism.
  • Gram stain: A method of staining bacterial cells while viewing them under a microscope.

See Also

External links

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