Polyacrylamide gel electrophoresis

Polyacrylamide Gel Electrophoresis (pronunciation: pol-ee-uh-kril-am-ide jel ih-lek-troh-foh-ree-sis) is a technique widely used in life sciences for the separation of biological macromolecules such as proteins and nucleic acids in an electric field.

Etymology

The term "Polyacrylamide Gel Electrophoresis" is derived from the main components and process involved. "Polyacrylamide" refers to the type of gel used, "gel" refers to the medium of the process, and "electrophoresis" refers to the method of separating molecules based on their size and charge.

Process

Polyacrylamide Gel Electrophoresis involves the use of a polyacrylamide gel as a sieving medium during electrophoresis, the movement of charged particles in a fluid under the influence of an electric field. The technique is used to separate macromolecules such as proteins and nucleic acids.

Related Terms

• Electrophoresis
• Macromolecule
• Protein
• Nucleic Acid
• Electric Field

See Also

• Agarose Gel Electrophoresis
• Capillary Electrophoresis
• Isoelectric Focusing

External links

• Medical encyclopedia article on Polyacrylamide gel electrophoresis
• Wikipedia's article - Polyacrylamide gel electrophoresis

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