RT-PCR
| RT-PCR | |
|---|---|
| Term | RT-PCR |
| Short definition | RT-PCR - A laboratory method used to make many copies of a given genetic sequence for analysis. It uses an enzyme called reverse transcriptase to convert a specific piece of RNA into a matching piece of DNA. |
| Type | Cancer terms |
| Specialty | Oncology |
| Language | English |
| Source | NCI |
| Comments | |
RT-PCR - A laboratory method used to make many copies of a given genetic sequence for analysis. It uses an enzyme called reverse transcriptase to convert a specific piece of RNA into a matching piece of DNA. This piece of DNA is then amplified (made in large numbers) by another enzyme called DNA polymerase. The amplified DNA copies help determine whether a particular mRNA molecule is made by a gene. RT-PCR can be used to look for specific changes in a gene or chromosome, or for specific genes to be activated, which can help diagnose a disease such as cancer. It can also be used to examine the RNA of certain viruses, such as B. human immunodeficiency virus (HIV) and hepatitis C virus to help diagnose and monitor infection. Also called reverse transcription polymerase chain reaction
External links
- Medical encyclopedia article on RT-PCR
- Wikipedia's article - RT-PCR
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