Organ culture

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Organ Culture

Organ culture (pronunciation: /ˈɔːrɡən ˈkʌltʃər/) is a development from tissue culture methods of research, where whole organs are maintained in vitro in a suitable environment for a certain period of time.

Etymology

The term "organ culture" is derived from the Latin word "organum" meaning instrument, and the Middle English word "culture" meaning cultivation.

Definition

Organ culture is a method used in laboratory settings to maintain an organ in a way that may mimic in vivo conditions, allowing the organ to function as it would in the body. This is achieved by providing the organ with a suitable environment, including the necessary nutrients and conditions such as temperature and pH.

Methodology

The organ is first removed from the organism in a sterile environment to prevent contamination. It is then placed in a culture medium that provides the necessary nutrients and conditions for the organ to survive. The culture medium is often supplemented with serum, which provides additional nutrients and growth factors. The organ is then maintained in this environment for a certain period of time, during which its function and behavior can be studied.

Applications

Organ culture has a wide range of applications in biomedical research, including the study of organ development and function, the investigation of disease processes, and the testing of drug effects. It is also used in transplantation research, where it can help to improve organ preservation methods and increase the success rate of transplants.

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