Immunoelectrophoresis
Immunoelectrophoresis (pronounced: im·mu·no·e·lec·tro·pho·re·sis) is a laboratory method used in biochemistry, immunology, and other fields of medical science. The term is derived from the Greek words "immuno" (meaning immunity) and "electrophoresis" (meaning to carry with electricity).
Definition
Immunoelectrophoresis is a two-step procedure for both antigen detection and quantification. It involves the separation of serum proteins by electrophoresis, followed by immunodiffusion.
Procedure
The first step in immunoelectrophoresis is to separate the proteins in a sample by electrophoresis. This involves applying an electric field to a gel containing the sample, causing the proteins to move according to their charge and size. The second step is immunodiffusion, in which antibodies are added to the gel. These antibodies bind to specific proteins, forming visible precipitin lines that can be used to identify and quantify the proteins.
Applications
Immunoelectrophoresis is used in the diagnosis of various diseases, including multiple myeloma, Waldenstrom's macroglobulinemia, and other conditions that affect the immune system. It can also be used to detect and quantify specific proteins in a sample, such as immunoglobulins.
Related Terms
- Electrophoresis
- Immunodiffusion
- Antigen
- Antibody
- Serum
- Protein
- Immunoglobulins
- Multiple Myeloma
- Waldenstrom's macroglobulinemia
External links
- Medical encyclopedia article on Immunoelectrophoresis
- Wikipedia's article - Immunoelectrophoresis
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