English: Phylogenetic analysis of PRNTase and PRNTase-like domains in L proteins of non-segmented negative strand (NNS) RNA viruses and their related viruses. Amino acid sequences of core PRNTase and PRNTase-like domains of selected NNS RNA viral L proteins were aligned using the PSI-Coffee program3 (Di Tommaso et al., 2011). A phylogenetic analysis based on the alignment was performed using the Molecular Evolutionary Genetics Analysis (MEGA) software (version 7.0) (Kumar et al., 2016). A phylogenetic tree was generated by the Maximum Likelihood method with 1,000 bootstrap repetitions. Colored branches represent different families. Genus names are placed close to the virus names. The numbers at the nodes indicate bootstrap values. The scale bar shows the branch length corresponding to 0.5 amino acid substitutions per site. The domain organization of L proteins of NNS RNA viruses belonging to the indicated families is schematically represented.

Virus names (abbreviations, GenBank accession numbers, and amino acid residue ranges) are as follows:

• vesicular stomatitis Indiana virus (VSIV, K02378, 1081–1302)
• Chandipura virus (CHPV, AJ810083, 1071–1292)
• spring viremia of carp virus (SVCV, AJ318079, 1069–1290)
• pike fry rhabdovirus (PFRV, FJ872827, 1069–1290)
• perch rhabdovirus (PRV, JX679246, 1093–1310)
• eel virus European X (EVEX, FN557213, 1070–1287)
• Le Dantec virus (LDV, KM205006, 1085–1309)
• Barur virus (BARV, KM204983, 1080–1302)
• Tibrogargan virus (TIBV, GQ294472, 1098–1321)
• Bas-Congo virus (BASV, JX297815, 1097–1319)
• Durham virus (DURV, FJ952155, 1075–1296)
• tupaia virus (TUPV, AY840978, 1077–1297)
• Flanders virus (FLAV, AF523199, 1097–1318)
• Hart Park virus (HPV, KM205011, 1098–1319)
• Drosophila melanogaster sigmavirus (DMelSV, GQ375258, 1084–1307)
• Drosophila obscura sigmavirus (DObsSV, GQ410979, 1100–1323)
• Niakha virus (NIAV, KC585008, 1084–1306)
• Sripur virus (SRIV, KM205023, 1083–1305)
• Curionopolis virus (CURV, KJ701190, 1118–1338)
• Iriri virus (IRIRV, KM204995, 1113–1333)
• bovine ephemeral fever virus (BEFV, AF234533, 1108–1331)
• Adelaide River virus (ARV, JN935380, 1107–1329)
• Moussa virus (MOUV, FJ985748, 1101–1314)
• rabies virus (RABV, M13215, 1093–1320)
• Ikoma lyssavirus (IKOV, JX193798, 1093–1320)
• Puerto Almendras virus (PTAMV, KF534749, 1077–1298)
• Arboretum virus (ABTV, KC994644, 1077–1298)
• lettuce big-vein associated virus (LBVaV, AB075039, 1075–1286)
• lettuce necrotic yellows virus (LNYV, AJ867584, 1064–1294)
• northern cereal mosaic virus (NCMV, AB030277, 1058–1278)
• potato yellow dwarf virus (PYDV, GU734660, 1082–1304)
• sonchus yellow net virus (SYNV, L32603, 1142–1362)
• orchid fleck virus (OFV, AB244418, 1073–1286)
• coffee ringspot virus (CoRSV, KF812526, 1073–1276)
• infectious hematopoietic necrosis virus (IHNV, X89213, 1078–1300)
• viral hemorrhagic septicemia virus (VHSV, Y18263, 1076–1298)
• Measles virus (MeV, M20865, 1132–1370)
• canine distemper virus (CDV, Y09629, 1132–1370)
• Hendra virus (HeV, AF017149, 1191–1429)
• Nipah virus (NiV, AF212302, 1191–1429)
• Fer-de-Lance virus (FDLV, AY141760, 1127–1365)
• Atlantic salmon paramyxovirus (AsaPV, EF646380, 1135–1374)
• Sendai virus (SeV, X03614, 1132–1372)
• human parainfluenza virus 3 (HPIV-3, M21649, 1132–1372)
• mumps virus (MuV, D10575, 1142–1380)
• human parainfluenza virus 2 (HPIV-2, X57559, 1140–1378)
• Newcastle disease virus (NDV, AY262106, 1112–1350)
• avian paramyxovirus 2 (APMV-2, EU338414, 1144–1382)
• human respiratory syncytial virus-A2 (HRSV-A2, M75730, 1198–1419)
• murine pneumonia virus (MPV, AY729016, 1133–1354)
• avian metapneumovirus (AMPV, U65312, 1122–1343)
• human metapneumovirus (HMPV, AF371337, 1123–1344)
• Ebola virus (EBOV, AF086833, 1105–1352)
• Reston virus (RESTV, AF522874, 1105–1350)
• Marburg virus (MARV, Z29337, 1108–1377)
• Lloviu virus (LLOV, JF828358, 1102–1349)
• Borna disease virus 1 (BoDV-1, AJ311522, 993–1213)
• canary bornavirus 1 (CnBV-1, KC464471, 993–1213)
• jungle carpet python virus (JCPV, MF135780, 990–1209)
• southwest carpet python virus (SWCPV, MF135781, 991–1210)
• Wenzhou tapeworm virus 1 (WzTWV1, KX884436, 1030–1250)
• Wçnzhōu crab virus 1 (WzCV-1, KM817644, 985–1205)
• Beihai rhabdo-like virus 6 (BhLV-6, KX884405, 1009–1229)
• Orinoco virus (ONCV, KX257488, 1006–1227)
• soybean cyst nematode virus 1 (SbCNV-1, HM849038, 1035–1283)
• Bìihai rhabdo-like virus 3 (BhRLV-3, KX884408, 1009–1229)
• Bìihai rhabdo-like virus 4 (BhRLV-4, KX884406, 1023–1241)
• Midway virus (MIDWV, FJ554525, 1038–1261)
• Nyamanini virus (NYMV, FJ554526, 1038–1261)
• Líshí spider virus 2 (LsSV-2, KM817632, 1167–1404)
• Tachéng tick virus 6 (TcTV-6, KM817641, 1069–1293)
• Sunshine Coast virus (SunCV, JN192445, 1119–1346)
• Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1, KJ186782, 1078–1292)
• Soybean leaf-associated negative-stranded RNA virus 1 (SLaNSRV-1, KT598225, 1090–1304)
• Pteromalus puparum negative-strand RNA virus 1 (PpNSRV-1, KX431032, 1034–1258)
• Bìihai barnacle virus 8 (BhBV-8, KX884410, 1100–1324)
• Xînchéng mosquito virus (XcMV, KM817661, 1048–1278)
• Bolahun virus (BLHV, KX148552, 1046–1277)
• Bìihai barnacle virus 9 (BhBV-9, KX884409, 1073–1320)
• Imjin River virus 1 (IjRV-1, KU095839, 1038–1279)
• Shāyáng fly virus 1 (SyFV-1, KM817598, 1114–1364), and
• Bìihai hermit crab virus 3 (BhHCV-3, KX884404, 1091–1342).