File:3D-image of mouse jejunum tuft cells.webm

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3D-image_of_mouse_jejunum_tuft_cells.webm(file size: 19.36 MB, MIME type: video/webm)

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English: Tuft cells in mouse jejunum: Tuft cells are epithelial cells with unknown function residing a broad spectrum of mammalian digestive tract, comprising 1% of whole epithelial cells in mouse jejunum. A tuft cell has a pointed apex and a spool-shaped soma. A free-floating cryosection was immunostained with a tuft cell marker (anti-phospho-specific antibody agaist Girdin tyrosine-1798; pY1798 antibody) following an establised method (Kuga D et al. Journal of Histochemistry & Cytochemistry 65(6) 347-366, Mizutani Y et al. Journal of Visualized Experiments (133) e57475). Tuft cells are receiving a lot more attention lately, as its involvement in parasite infection or in carcinogenesis. SAMPLE: Cryosectioned free-floating DDY mouse jejunum (green: phospho-Girdin at tyrosine 1798, red: phalloidin, blue: DAPI) prepared by Iida M, Tanaka M, Asai M in Institute for Developmental Research, Aichi Human Service Center (Kasugai Japan). 3D-video edited by Ito T in Nikon Instech. MICROSCOPE: NIKON A1R-TiE. OBJECTIVE LENS: Plan Apo λ 60x Oil.
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Author Masato Asai

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11 June 2018

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current08:31, 24 June 2018 (19.36 MB)Masato Asaihigher resolution

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