Eluent
Eluent
Eluent (pronounced: /ɪˈluːənt/), also known as eluant or mobile phase, is a term used in Chromatography to describe the liquid or gas that carries the Analyte through the Chromatographic column. The term "eluent" is derived from the Latin word "eluere", which means "to wash out".
Usage
In chromatography, the eluent is the substance that carries the analyte through the chromatographic column. The choice of eluent can greatly affect the efficiency of the separation process, as it can influence the interaction between the analyte and the Stationary phase.
Types of Eluents
Eluents can be broadly classified into two types: Polar and Nonpolar. Polar eluents, such as water or methanol, are used for Reverse phase chromatography, while nonpolar eluents, such as hexane or chloroform, are used for Normal phase chromatography.
Related Terms
- Elution: The process of extracting one material from another by washing with a solvent.
- Eluate: The liquid obtained from the process of elution.
- Elutriation: A process of separating particles based on their size, shape and density.
External links
- Medical encyclopedia article on Eluent
- Wikipedia's article - Eluent
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