Signature-tagged mutagenesis
Signature-tagged mutagenesis (STM) is a genetic approach used to study gene function. This technique was developed to identify novel bacterial virulence factors.
Technique
It is a negative selection method in which unique identification tags that allow analysis of pools of mutants in mixed populations.
Understanding the function
Recent advances in genome sequencing have allowed us to catalogue a large variety of organisms' genomes, but the function of the genes they contain is still largely unknown. Using STM, the function of the product of a particular gene can be inferred by disabling it and observing the effect on the organism.
Uses
The most important use of STM is to discover which genes in a pathogen are involved in virulence in its host, to aid the development of new medical therapies/drugs.
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