Vero cell

Vero Cell

Vero cells under a microscope

Vero cells are a type of cell line derived from the kidney of the African green monkey, Chlorocebus sabaeus. These cells are widely used in cell culture and virology research due to their ability to support the growth of a wide range of viruses.

History

Vero cells were first isolated in 1962 by Yasumura and Kawakita at the Chiba University in Japan. The name "Vero" is derived from "Verda Reno," which means "green kidney" in Esperanto, reflecting their origin from the kidney of the African green monkey.

Characteristics

Vero cells are epithelial cells and are characterized by their fibroblast-like morphology. They are adherent cells, meaning they require a surface to attach to in order to grow. Vero cells are anchorage-dependent, which makes them suitable for use in monolayer cultures.

Applications

Vero cells are extensively used in the production of vaccines, including those for polio, rabies, and smallpox. They are also used in the study of viral pathogenesis and toxicology.

Vaccine Production

Vero cells are a preferred substrate for vaccine production because they are capable of producing high yields of virus. This makes them ideal for the production of inactivated vaccines and live attenuated vaccines.

Virology Research

In virology, Vero cells are used to study the replication and pathogenesis of various viruses, including SARS-CoV-2, the virus responsible for COVID-19. Their ability to support the growth of a wide range of viruses makes them a valuable tool in the development of antiviral drugs.

Advantages and Limitations

Advantages

• Versatility: Vero cells can support the growth of many different types of viruses.
• Consistency: They provide a consistent and reproducible system for virus production.
• Regulatory Approval: Vero cells are well-characterized and have been approved by regulatory agencies for vaccine production.

Limitations

• Species Origin: As they are derived from monkey cells, there is a risk of contamination with simian viruses.
• Genetic Stability: Over time, Vero cells can undergo genetic changes that may affect their characteristics.

Related Pages

• Cell culture
• Vaccine production
• Virology
• African green monkey