Bacillus virus phi29

Bacillus virus phi29 is a bacteriophage that infects the bacteria Bacillus subtilis. It is a member of the Podoviridae family, characterized by its short, non-contractile tail. The virus has been extensively studied due to its unique DNA replication mechanism and the high fidelity of its DNA polymerase, which has applications in nanotechnology and molecular biology.

Structure

Bacillus virus phi29 is composed of a icosahedral head and a short tail. The head encloses the viral DNA, which is a linear, double-stranded molecule. The phi29 DNA polymerase, responsible for replicating the virus's DNA, is highly processive and has proofreading capabilities, making it a valuable tool in DNA sequencing and amplification techniques.

Life Cycle

The life cycle of Bacillus virus phi29 begins with the attachment of the virus to specific receptor sites on the surface of Bacillus subtilis. Following attachment, the virus injects its DNA into the host cell. Inside the host, the viral DNA hijacks the cell's machinery to begin synthesizing viral components. New virus particles are assembled and eventually released from the cell, typically leading to cell lysis.

Applications

1. 1. 1. DNA Polymerase

The phi29 DNA polymerase is widely used in biotechnology for DNA amplification in the polymerase chain reaction (PCR) and DNA sequencing. Its high fidelity and ability to amplify long strands of DNA make it particularly useful in various molecular biology applications.

1. 1. 1. Nanotechnology

The precise assembly of phi29 and its components has inspired applications in nanotechnology, particularly in the development of nanomotors and nanodevices. The motor protein that packs DNA into the virus's capsid has been studied for its potential use in nano-scale assembly and transport systems.

Research

Research on Bacillus virus phi29 has contributed significantly to our understanding of viral DNA packaging, replication, and the mechanics of motor proteins. Studies have also explored the virus's potential in gene therapy and targeted drug delivery, leveraging its ability to specifically infect Bacillus subtilis.

See Also

• Bacteriophage
• DNA polymerase
• Nanotechnology in medicine
• Gene therapy

References

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  Bacillus phage phi29
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  Bacteriophage phi29
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  Phi29likevirus virion
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  Targeting of TNBC molecules by bacteriophage phi29 pRNA