LNCaP: Difference between revisions

Revision as of 20:24, 30 December 2024

LNCaP is a human cell line commonly used in cancer research, particularly in the study of prostate cancer. This cell line was derived from a lymph node metastasis of a human prostatic adenocarcinoma.

Characteristics

LNCaP cells are known for their sensitivity to androgens, which are hormones that play a crucial role in the development and maintenance of male characteristics. This makes them an important model for studying androgen receptor signaling and the effects of androgen deprivation therapy in prostate cancer.

Applications

LNCaP cells are widely used in drug discovery and biomedical research to test the efficacy of new therapeutic agents targeting prostate cancer. They are also used to study the mechanisms of cancer progression and metastasis.

Culture Conditions

LNCaP cells are typically cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and maintained at 37°C in a humidified atmosphere containing 5% carbon dioxide.

References

External Links

[LNCaP cell line information at ATCC](https://www.atcc.org/products/all/CRL-1740.aspx)

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-[[File:LNCaP.jpg|thumb]] [[File:Comparison of cancer cell lines.png|thumb]] [[File:Thalmann figure.png|thumb]] {{Short description|A human prostate cancer cell line used in research}}
-'''LNCaP''' is a human [[prostate cancer]] cell line commonly used in [[cancer research]]. It was established from a metastatic lesion of human prostatic adenocarcinoma in the left supraclavicular lymph node of a 50-year-old Caucasian male. LNCaP cells are androgen-sensitive, meaning they require [[androgens]] for growth, which makes them a valuable model for studying [[androgen receptor]] signaling and prostate cancer progression.
+{{Infobox cell line
+| name = LNCaP
+| image = <!-- Image removed -->
+| caption = <!-- Caption removed -->
+| type = [[Human cell line]]
+| origin = [[Prostate cancer]]
+| characteristics = Androgen-sensitive
+| applications = [[Cancer research]], [[Drug discovery]]
+}}
-==Characteristics==
+'''LNCaP''' is a [[human cell line]] commonly used in [[cancer research]], particularly in the study of [[prostate cancer]]. This cell line was derived from a [[lymph node]] metastasis of a [[human]] [[prostatic adenocarcinoma]].
-LNCaP cells exhibit several key characteristics that make them useful for research:
-* '''Androgen Sensitivity''': LNCaP cells express the [[androgen receptor]] (AR) and require androgens for optimal growth. This property is crucial for studying the role of androgens in prostate cancer.
+== Characteristics ==
+LNCaP cells are known for their sensitivity to [[androgens]], which are [[hormones]] that play a crucial role in the development and maintenance of [[male characteristics]]. This makes them an important model for studying [[androgen receptor]] signaling and the effects of [[androgen deprivation therapy]] in prostate cancer.
-* '''PSA Production''': LNCaP cells produce [[prostate-specific antigen]] (PSA), a protein commonly used as a biomarker for prostate cancer diagnosis and monitoring.
+== Applications ==
+LNCaP cells are widely used in [[drug discovery]] and [[biomedical research]] to test the efficacy of new [[therapeutic agents]] targeting prostate cancer. They are also used to study the mechanisms of [[cancer progression]] and [[metastasis]].
-* '''Genetic Profile''': LNCaP cells have a mutation in the AR gene, which affects their response to androgens and anti-androgens. This mutation is often studied to understand resistance mechanisms to [[androgen deprivation therapy]].
+== Culture Conditions ==
+LNCaP cells are typically cultured in [[RPMI 1640 medium]] supplemented with 10% [[fetal bovine serum]] (FBS) and maintained at 37°C in a humidified atmosphere containing 5% [[carbon dioxide]].
-* '''Growth Characteristics''': LNCaP cells grow as adherent monolayers and have a doubling time of approximately 60 hours under standard culture conditions.
+== See Also ==
-==Applications in Research==
-LNCaP cells are widely used in various areas of prostate cancer research:
-* '''Drug Testing''': Researchers use LNCaP cells to test the efficacy of new drugs and therapies targeting the androgen receptor pathway.
-* '''Mechanistic Studies''': The cell line is used to study the molecular mechanisms of prostate cancer progression, including the role of androgens and the AR.
-* '''Biomarker Discovery''': LNCaP cells help in identifying potential biomarkers for prostate cancer diagnosis and prognosis.
-* '''Resistance Mechanisms''': The cell line is used to investigate mechanisms of resistance to androgen deprivation therapy and other treatments.
-==Limitations==
-While LNCaP cells are a valuable tool, they have limitations:
-* '''Genetic Drift''': Over time, LNCaP cells can undergo genetic changes that may affect experimental outcomes.
-* '''Limited Heterogeneity''': As a single cell line, LNCaP does not capture the genetic and phenotypic diversity of prostate cancer in patients.
-* '''Androgen Dependence''': The androgen-sensitive nature of LNCaP cells may not fully represent castration-resistant prostate cancer.
-==Also see==
 * [[Prostate cancer]]
 * [[Androgen receptor]]
-* [[Prostate-specific antigen]]
-* [[Androgen deprivation therapy]]
 * [[Cancer cell line]]
-{{Prostate cancer}}
+== References ==
-{{Cell lines}}
+<references />
+== External Links ==
+* [LNCaP cell line information at ATCC](https://www.atcc.org/products/all/CRL-1740.aspx)
+[[Category:Cell lines]]
 [[Category:Prostate cancer]]
-[[Category:Cell lines]]
 [[Category:Cancer research]]