LNCaP: Difference between revisions

Revision as of 15:26, 9 December 2024

File:Comparison of cancer cell lines.png

A human prostate cancer cell line used in research

LNCaP is a human prostate cancer cell line commonly used in cancer research. It was established from a metastatic lesion of human prostatic adenocarcinoma in the left supraclavicular lymph node of a 50-year-old Caucasian male. LNCaP cells are androgen-sensitive, meaning they require androgens for growth, which makes them a valuable model for studying androgen receptor signaling and prostate cancer progression.

Characteristics

LNCaP cells exhibit several key characteristics that make them useful for research:

Androgen Sensitivity: LNCaP cells express the androgen receptor (AR) and require androgens for optimal growth. This property is crucial for studying the role of androgens in prostate cancer.

PSA Production: LNCaP cells produce prostate-specific antigen (PSA), a protein commonly used as a biomarker for prostate cancer diagnosis and monitoring.

Genetic Profile: LNCaP cells have a mutation in the AR gene, which affects their response to androgens and anti-androgens. This mutation is often studied to understand resistance mechanisms to androgen deprivation therapy.

Growth Characteristics: LNCaP cells grow as adherent monolayers and have a doubling time of approximately 60 hours under standard culture conditions.

Applications in Research

LNCaP cells are widely used in various areas of prostate cancer research:

Drug Testing: Researchers use LNCaP cells to test the efficacy of new drugs and therapies targeting the androgen receptor pathway.

Mechanistic Studies: The cell line is used to study the molecular mechanisms of prostate cancer progression, including the role of androgens and the AR.

Biomarker Discovery: LNCaP cells help in identifying potential biomarkers for prostate cancer diagnosis and prognosis.

Resistance Mechanisms: The cell line is used to investigate mechanisms of resistance to androgen deprivation therapy and other treatments.

Limitations

While LNCaP cells are a valuable tool, they have limitations:

Genetic Drift: Over time, LNCaP cells can undergo genetic changes that may affect experimental outcomes.

Limited Heterogeneity: As a single cell line, LNCaP does not capture the genetic and phenotypic diversity of prostate cancer in patients.

Androgen Dependence: The androgen-sensitive nature of LNCaP cells may not fully represent castration-resistant prostate cancer.

Also see

Template:Cell lines

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-[[file:LNCaP.jpg|thumb|LNCaP]] [[file:Comparison_of_cancer_cell_lines.png|thumb|Comparison of cancer cell lines|left]] [[file:Thalmann_figure.png|thumb|Thalmann figure]] '''LNCaP''' (Lymph Node Carcinoma of the Prostate) is a human [[prostate cancer]] cell line commonly used in [[cancer research]]. It was established from a metastatic lesion of human prostatic adenocarcinoma in the left supraclavicular lymph node of a 50-year-old Caucasian male. LNCaP cells are androgen-sensitive, meaning their growth is stimulated by [[androgens]], which are male hormones such as [[testosterone]].
+[[File:LNCaP.jpg|thumb]] [[File:Comparison of cancer cell lines.png|thumb]] [[File:Thalmann figure.png|thumb]] {{Short description|A human prostate cancer cell line used in research}}
+'''LNCaP''' is a human [[prostate cancer]] cell line commonly used in [[cancer research]]. It was established from a metastatic lesion of human prostatic adenocarcinoma in the left supraclavicular lymph node of a 50-year-old Caucasian male. LNCaP cells are androgen-sensitive, meaning they require [[androgens]] for growth, which makes them a valuable model for studying [[androgen receptor]] signaling and prostate cancer progression.
 ==Characteristics==
-LNCaP cells exhibit several characteristics that make them valuable for research:
+LNCaP cells exhibit several key characteristics that make them useful for research:
-* They express [[prostate-specific antigen]] (PSA), a protein produced by both normal and malignant cells of the prostate gland.
-* They possess a mutated [[androgen receptor]] (AR), which allows them to respond to lower levels of androgens compared to normal prostate cells.
+* '''Androgen Sensitivity''': LNCaP cells express the [[androgen receptor]] (AR) and require androgens for optimal growth. This property is crucial for studying the role of androgens in prostate cancer.
-* They are used to study the mechanisms of [[androgen receptor]] signaling and the development of [[androgen-independent prostate cancer]].
+* '''PSA Production''': LNCaP cells produce [[prostate-specific antigen]] (PSA), a protein commonly used as a biomarker for prostate cancer diagnosis and monitoring.
-==Research Applications==
+* '''Genetic Profile''': LNCaP cells have a mutation in the AR gene, which affects their response to androgens and anti-androgens. This mutation is often studied to understand resistance mechanisms to [[androgen deprivation therapy]].
-LNCaP cells are widely used in various areas of prostate cancer research, including:
-* [[Drug discovery]]: Testing the efficacy of new therapeutic agents.
-* [[Gene expression]] studies: Understanding the role of specific genes in prostate cancer progression.
-* [[Signal transduction]] pathways: Investigating how signals are transmitted within cells to promote cancer growth.
-==Culturing LNCaP Cells==
+* '''Growth Characteristics''': LNCaP cells grow as adherent monolayers and have a doubling time of approximately 60 hours under standard culture conditions.
-LNCaP cells are typically cultured in [[RPMI-1640]] medium supplemented with 10% [[fetal bovine serum]] (FBS) and 1% [[penicillin-streptomycin]]. They are maintained at 37°C in a humidified atmosphere containing 5% [[carbon dioxide]] (CO2).
-==Related Cell Lines==
+==Applications in Research==
-Other prostate cancer cell lines used in research include:
+LNCaP cells are widely used in various areas of prostate cancer research:
-* [[PC-3]]
-* [[DU145]]
-* [[22Rv1]]
-==See Also==
+* '''Drug Testing''': Researchers use LNCaP cells to test the efficacy of new drugs and therapies targeting the androgen receptor pathway.
+* '''Mechanistic Studies''': The cell line is used to study the molecular mechanisms of prostate cancer progression, including the role of androgens and the AR.
+* '''Biomarker Discovery''': LNCaP cells help in identifying potential biomarkers for prostate cancer diagnosis and prognosis.
+* '''Resistance Mechanisms''': The cell line is used to investigate mechanisms of resistance to androgen deprivation therapy and other treatments.
+==Limitations==
+While LNCaP cells are a valuable tool, they have limitations:
+* '''Genetic Drift''': Over time, LNCaP cells can undergo genetic changes that may affect experimental outcomes.
+* '''Limited Heterogeneity''': As a single cell line, LNCaP does not capture the genetic and phenotypic diversity of prostate cancer in patients.
+* '''Androgen Dependence''': The androgen-sensitive nature of LNCaP cells may not fully represent castration-resistant prostate cancer.
+==Also see==
 * [[Prostate cancer]]
 * [[Androgen receptor]]
 * [[Prostate-specific antigen]]
+* [[Androgen deprivation therapy]]
 * [[Cancer cell line]]
-* [[Drug discovery]]
-==References==
+{{Prostate cancer}}
-{{Reflist}}
+{{Cell lines}}
-==External Links==
-{{Commons category|LNCaP}}
 [[Category:Prostate cancer]]
 [[Category:Cell lines]]
 [[Category:Cancer research]]
-[[Category:Medical research]]
-{{medicine-stub}}