Renaturation
Renaturation
Renaturation (pronunciation: /ˌriːnætʃʊˈreɪʃən/) is a process in molecular biology that involves the conversion of denatured protein or DNA back to its original structure.
Etymology
The term "renaturation" is derived from the Latin prefix re-, meaning "again", and the English word nature. It signifies the restoration of the natural state of a substance.
Process
Renaturation of DNA occurs when the two separate strands of a DNA molecule reanneal to form the original double helix structure. This process is facilitated by slowly cooling the denatured DNA.
In the case of proteins, renaturation involves the refolding of the protein into its original three-dimensional structure. This is often achieved by removing the denaturing agent and allowing the protein to spontaneously refold.
Related Terms
- Denaturation: The process that alters the natural qualities of a substance, often referring to proteins or DNA.
- Protein folding: The physical process by which a protein chain acquires its native 3-dimensional structure.
- Molecular biology: The branch of biology that deals with the molecular basis of biological activity.
See Also
External links
- Medical encyclopedia article on Renaturation
- Wikipedia's article - Renaturation
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