Perls Prussian blue
Perls Prussian Blue
Perls Prussian Blue (pronunciation: /pɛrlz ˈprʌʃən bluː/) is a histochemical stain used primarily in the field of pathology. It is named after the German pathologist Max Perls, who developed the technique in the late 19th century.
Etymology
The term "Perls Prussian Blue" is derived from the name of its developer, Max Perls, and the distinctive blue color of the stain, which is reminiscent of the color known as Prussian blue in the art world. Prussian blue, in turn, gets its name from the Kingdom of Prussia, where it was first synthesized in the early 18th century.
Usage
Perls Prussian Blue is used to detect the presence of iron in tissue samples. It is particularly useful in identifying hemosiderin, a form of iron storage complex, and ferritin, an intracellular iron-storage protein. The stain turns blue in the presence of ferric iron (Fe3+).
Procedure
The procedure for applying Perls Prussian Blue involves several steps. First, the tissue sample is fixed and sectioned. The sections are then treated with a solution of hydrochloric acid and potassium ferrocyanide, which reacts with ferric iron to form a blue precipitate. The sections are then counterstained, usually with nuclear fast red, to provide contrast.
Related Terms
- Histochemistry: The study of the chemical composition of cells and tissues.
- Pathology: The study of disease, its causes, processes, development, and consequences.
- Hemosiderin: An iron-storage complex, usually found within cells in a form that is not easily accessible to participate in chemical reactions.
- Ferritin: A protein that stores iron and releases it in a controlled fashion.
- Hydrochloric acid: A strong, corrosive acid that is commonly used in laboratory settings.
- Potassium ferrocyanide: A compound that forms a blue precipitate in the presence of ferric iron.
- Nuclear fast red: A counterstain used in histology to provide contrast to the blue color of Perls Prussian Blue.
External links
- Medical encyclopedia article on Perls Prussian blue
- Wikipedia's article - Perls Prussian blue
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