Luxol fast blue stain
Luxol Fast Blue Stain
Luxol fast blue stain (pronunciation: luks-ol fast bloo steyn) is a commonly used staining method in histology and pathology. It is primarily used to stain myelin, a lipid-rich substance that surrounds nerve fibers and aids in the conduction of nerve impulses.
Etymology
The term "Luxol fast blue" is derived from the name of the dye used in the staining process. The dye is known for its ability to bind to lipids, which are abundant in myelin. The term "fast" refers to the dye's resistance to fading or washing out, while "blue" refers to the color of the stain.
Procedure
The Luxol fast blue stain procedure involves several steps. First, tissue sections are immersed in a solution of Luxol fast blue dye. The sections are then heated to facilitate the binding of the dye to the myelin. After staining, the sections are differentiated using an alcohol solution, which removes excess dye. The sections are then counterstained, often with cresyl violet, to stain the Nissl substance in neurons.
Applications
Luxol fast blue stain is widely used in the study of various neurological disorders, such as multiple sclerosis, where it can reveal areas of myelin loss. It is also used in research to study the structure and function of the nervous system.
Related Terms
- Histology: The study of the microscopic structure of tissues.
- Pathology: The study of disease, its causes, processes, development, and consequences.
- Myelin: A lipid-rich substance that surrounds nerve fibers and aids in the conduction of nerve impulses.
- Cresyl violet: A dye used in histology for the staining of Nissl substance in neurons.
- Nissl substance: A large granular body found in neurons. These granules are of rough endoplasmic reticulum with rosettes of free ribosomes, and are the site of protein synthesis.
External links
- Medical encyclopedia article on Luxol fast blue stain
- Wikipedia's article - Luxol fast blue stain
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