Directed mutagenesis
Directed Mutagenesis
Directed mutagenesis (pronunciation: dih-rekted myoo-tuh-juh-ne-sis) is a molecular biology technique used to make specific and intentional changes to the DNA sequence of a gene and any gene products. Also known as site-directed mutagenesis or site-specific mutagenesis, this method is used to investigate the structure and biological function of DNA, RNA, and protein molecules.
Etymology
The term "Directed Mutagenesis" is derived from the English words "directed" and "mutagenesis". "Directed" means to control the course or direction of an action. "Mutagenesis" is derived from the Latin word "mutare", meaning to change, and the Greek word "genesis", meaning origin. Thus, "Directed Mutagenesis" refers to the controlled change in the origin, specifically in the genetic material of an organism.
Procedure
Directed mutagenesis involves the alteration of a specific DNA sequence. The process begins with the synthesis of a DNA strand that is complementary to the gene of interest, but with the desired mutation. This is followed by the amplification of this mutated strand via PCR. The amplified DNA is then introduced into a host organism, where it replaces the original, unmutated gene.
Applications
Directed mutagenesis has a wide range of applications in molecular biology, including the study of protein function, the creation of genetically modified organisms, and the development of new drugs and therapies. It is also used in protein engineering to improve the properties of proteins for industrial applications.
Related Terms
See Also
External links
- Medical encyclopedia article on Directed mutagenesis
- Wikipedia's article - Directed mutagenesis
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