Monospot test

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Monospot Test

The Monospot Test (pronunciation: /ˈmɒnəˌspɒt tɛst/), also known as the Heterophile Antibody Test or Paul-Bunnell Test, is a form of blood test commonly used to diagnose infectious mononucleosis (mono). The term "Monospot" is derived from the Greek words "monos" meaning "single" and "spot" referring to the distinctive reaction observed in the test.

Procedure

The Monospot Test involves the detection of heterophile antibodies in the blood. These antibodies are produced by the immune system in response to an infection by the Epstein-Barr virus, the most common cause of mono. The test is typically performed by taking a blood sample from a vein in the arm.

Interpretation

A positive Monospot Test indicates the presence of heterophile antibodies and, therefore, a likely infection with the Epstein-Barr virus. However, a negative test does not necessarily rule out mono, as it can take between two to nine weeks after infection for the antibodies to be detectable. In such cases, other tests may be required for a definitive diagnosis.

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