Immunophenotyping

From WikiMD's medical encyclopedia

(Redirected from Immunophenotype)

Pronounced IH-myoo-noh-FEE-noh-ty-ping, it is a process that uses antibodies to identify cells based on the types of antigens or markers on the surface of the cells.

Burkitt Lymphoma (FNA, Giemsa stain) (4679964756) On immunophenotyping by flow cytometry this FNA specimen was positive for CD10, CD20, and kappa sIg; negative for CD5 and lambda sIg.
Burkitt Lymphoma

Use in medicine

This process is used to diagnose specific types of leukemia and lymphoma by comparing the cancer cells to normal cells of the immune system.

Markers

Immunophenotyping may also be used to separate cells into different groups based on the markers they have on the surface.

Flow cytometry

It is a common flow cytometry test where fluorophore-conjugated antibodies are used as probes for staining target cells with high avidity and affinity.

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Contributors: Prab R. Tumpati, MD