Embryonic stem cell: Difference between revisions
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Embryonic stem cells are characterized by their ability to differentiate into any cell type of the three [[germ layers]]: [[ectoderm]], [[endoderm]], and [[mesoderm]]. This pluripotency distinguishes them from [[multipotent]] or [[unipotent]] stem cells, which can only form a limited range of cell types. | Embryonic stem cells are characterized by their ability to differentiate into any cell type of the three [[germ layers]]: [[ectoderm]], [[endoderm]], and [[mesoderm]]. This pluripotency distinguishes them from [[multipotent]] or [[unipotent]] stem cells, which can only form a limited range of cell types. | ||
[[File:MESC_EBs.jpg|thumb|Mouse embryonic stem cell-derived embryoid bodies]] | |||
ESCs are also capable of [[self-renewal]], meaning they can divide and replicate indefinitely while maintaining their undifferentiated state. This property is crucial for their potential use in [[regenerative medicine]] and [[tissue engineering]]. | ESCs are also capable of [[self-renewal]], meaning they can divide and replicate indefinitely while maintaining their undifferentiated state. This property is crucial for their potential use in [[regenerative medicine]] and [[tissue engineering]]. | ||
==Derivation== | ==Derivation== | ||
The derivation of embryonic stem cells involves isolating the inner cell mass from a blastocyst and culturing these cells in vitro. This process requires precise conditions to maintain the cells' pluripotency and prevent spontaneous differentiation. | The derivation of embryonic stem cells involves isolating the inner cell mass from a blastocyst and culturing these cells in vitro. This process requires precise conditions to maintain the cells' pluripotency and prevent spontaneous differentiation. | ||
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==Embryoid Bodies== | ==Embryoid Bodies== | ||
When cultured in suspension, embryonic stem cells can form [[embryoid bodies]], which are three-dimensional aggregates that can differentiate into various cell types, mimicking early embryonic development. | When cultured in suspension, embryonic stem cells can form [[embryoid bodies]], which are three-dimensional aggregates that can differentiate into various cell types, mimicking early embryonic development. | ||
Latest revision as of 18:37, 21 February 2025
Embryonic Stem Cell
Embryonic stem cells (ESCs) are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage pre-implantation embryo. Human embryos reach the blastocyst stage 4–5 days post fertilization, at which point they consist of 50–150 cells.
Characteristics[edit]
Embryonic stem cells are characterized by their ability to differentiate into any cell type of the three germ layers: ectoderm, endoderm, and mesoderm. This pluripotency distinguishes them from multipotent or unipotent stem cells, which can only form a limited range of cell types.
ESCs are also capable of self-renewal, meaning they can divide and replicate indefinitely while maintaining their undifferentiated state. This property is crucial for their potential use in regenerative medicine and tissue engineering.
Derivation[edit]
The derivation of embryonic stem cells involves isolating the inner cell mass from a blastocyst and culturing these cells in vitro. This process requires precise conditions to maintain the cells' pluripotency and prevent spontaneous differentiation.
Applications[edit]
Embryonic stem cells hold significant promise for therapeutic cloning and regenerative medicine. They can potentially be used to generate tissues and organs for transplantation, model diseases, and screen for new pharmaceuticals.
Ethical Considerations[edit]
The use of embryonic stem cells raises ethical concerns, primarily because the process of deriving these cells involves the destruction of a human embryo. This has led to debates about the moral status of the embryo and the ethical implications of using ESCs in research and therapy.
Research and Discoveries[edit]
The pioneering work of scientists such as Martin Evans, who was awarded the Nobel Prize for his contributions to the field, has paved the way for advancements in understanding and utilizing embryonic stem cells.
Induced Pluripotent Stem Cells[edit]
Research into induced pluripotent stem cells (iPSCs) has provided an alternative to embryonic stem cells. iPSCs are generated by reprogramming adult cells to a pluripotent state, thus avoiding the ethical issues associated with ESCs.
Embryoid Bodies[edit]
When cultured in suspension, embryonic stem cells can form embryoid bodies, which are three-dimensional aggregates that can differentiate into various cell types, mimicking early embryonic development.
