Coomassie brilliant blue

Coomassie Brilliant Blue is a synthetic dye used primarily in the staining of proteins during electrophoresis. It is named after the town of Coomassie in Ghana, where the British first discovered the dye. The dye is also known as Brilliant Blue G, Brilliant Blue R, Acid Blue 90, and Coomassie Blue.

History

The dye was first synthesized in the late 19th century and has been used in various scientific applications since then. It was named after the town of Coomassie in Ghana, where the British first discovered the dye.

Uses

Coomassie Brilliant Blue is most commonly used in the staining of proteins during electrophoresis. It is also used in the Bradford protein assay, a colorimetric assay used to measure the concentration of total protein in a sample.

Properties

Coomassie Brilliant Blue is a synthetic dye that is blue in color. It has a molecular weight of 825.98 g/mol and a melting point of 200 °C. The dye is soluble in water and ethanol, but insoluble in most organic solvents.

Safety

Coomassie Brilliant Blue is considered safe for use in laboratory settings. However, it should be handled with care as it can cause skin and eye irritation. It is also harmful if swallowed or inhaled.

See also

• Protein electrophoresis
• Bradford protein assay
• Dye
• Synthetic dye

References

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