Acid fuchsin: Difference between revisions
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{{ | {{DISPLAYTITLE:Acid fuchsin}} | ||
'''Acid fuchsin''' is a | ==Acid fuchsin== | ||
[[File:Acid fuchsin.svg|thumb|right|Chemical structure of Acid fuchsin]] | |||
'''Acid fuchsin''', also known as '''acid magenta''', is a mixture of [[sulfonated]] derivatives of [[rosaniline]] and [[pararosaniline]]. It is a dark red synthetic dye used in various staining techniques in [[histology]] and [[bacteriology]]. | |||
==Chemical | ==Chemical Properties== | ||
Acid fuchsin is a | Acid fuchsin is a member of the [[triarylmethane dye]] family. It is soluble in water and alcohol, forming a red solution. The dye is characterized by its ability to bind to [[acidic]] components of tissues, making it useful for staining [[cytoplasm]], [[collagen]], and other tissue elements. | ||
==Applications in | ==Applications in Histology== | ||
In [[histology]], acid fuchsin is commonly used in the [[Masson's trichrome stain]], where it stains [[ | In [[histology]], acid fuchsin is commonly used in the [[Masson's trichrome stain]], where it stains [[muscle]] fibers red. It is also used in the [[Van Gieson's stain]] to differentiate between [[collagen]] and other tissue elements. Acid fuchsin is a component of the [[Mallory's trichrome stain]], which is used to highlight connective tissue. | ||
== | ==Applications in Bacteriology== | ||
In [[bacteriology]], acid fuchsin is used in the [[Ziehl-Neelsen stain]], a method for identifying [[acid-fast bacteria]], such as [[Mycobacterium tuberculosis]]. The dye penetrates the waxy cell wall of these bacteria, allowing them to be visualized under a microscope. | |||
== | ==Safety and Handling== | ||
[[File:Acid fuchsin.svg|thumb|left|Structural formula of Acid fuchsin]] | |||
As with many chemical dyes, acid fuchsin should be handled with care. It can cause skin and eye irritation, and appropriate [[personal protective equipment]] should be worn when handling the dye. It should be stored in a cool, dry place, away from incompatible substances. | |||
As with many chemical dyes, acid fuchsin should be handled with care. It | |||
==Related pages== | ==Related pages== | ||
* [[Histology]] | * [[Histology]] | ||
* [[ | * [[Bacteriology]] | ||
* [[Staining (biology)]] | * [[Staining (biology)]] | ||
* [[ | * [[Triarylmethane dye]] | ||
[[Category:Histology stains]] | [[Category:Histology stains]] | ||
[[Category: | [[Category:Biological dyes]] | ||
[[Category: | [[Category:Triarylmethane dyes]] | ||
Latest revision as of 03:35, 13 February 2025
Acid fuchsin[edit]
Acid fuchsin, also known as acid magenta, is a mixture of sulfonated derivatives of rosaniline and pararosaniline. It is a dark red synthetic dye used in various staining techniques in histology and bacteriology.
Chemical Properties[edit]
Acid fuchsin is a member of the triarylmethane dye family. It is soluble in water and alcohol, forming a red solution. The dye is characterized by its ability to bind to acidic components of tissues, making it useful for staining cytoplasm, collagen, and other tissue elements.
Applications in Histology[edit]
In histology, acid fuchsin is commonly used in the Masson's trichrome stain, where it stains muscle fibers red. It is also used in the Van Gieson's stain to differentiate between collagen and other tissue elements. Acid fuchsin is a component of the Mallory's trichrome stain, which is used to highlight connective tissue.
Applications in Bacteriology[edit]
In bacteriology, acid fuchsin is used in the Ziehl-Neelsen stain, a method for identifying acid-fast bacteria, such as Mycobacterium tuberculosis. The dye penetrates the waxy cell wall of these bacteria, allowing them to be visualized under a microscope.
Safety and Handling[edit]
As with many chemical dyes, acid fuchsin should be handled with care. It can cause skin and eye irritation, and appropriate personal protective equipment should be worn when handling the dye. It should be stored in a cool, dry place, away from incompatible substances.
