FISH

FISH or Fluorescent in situ hybridization is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity. It was developed by biomedical researchers in the early 1980s to detect and localize the presence or absence of specific DNA sequences on chromosomes.

History

The FISH technique was first developed in the early 1980s by biomedical researchers. It has since been used extensively in research and clinical settings, with its applications expanding over time.

Procedure

The FISH procedure involves the use of fluorescent probes that bind to only those parts of the nucleic acid sequence with a high degree of sequence complementarity. These probes are then visualized and quantified using fluorescence microscopy.

Applications

FISH has a wide range of applications in both research and clinical settings. It is used in genetic counseling, medicine, and species identification. It can also be used to detect and localize the presence or absence of specific DNA sequences on chromosomes.

Advantages

One of the main advantages of FISH is its ability to detect and localize specific DNA sequences on chromosomes. This makes it a powerful tool for identifying chromosomal abnormalities and genetic mutations.

Disadvantages

Despite its advantages, FISH also has some limitations. For example, it requires a high degree of sequence complementarity between the probe and the target DNA sequence. This means that it may not be able to detect certain types of genetic mutations.

See also

• Molecular cytogenetics
• Fluorescence microscopy
• Genetic counseling
• Medicine
• Species identification

References

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