H1299: Difference between revisions

Latest revision as of 22:34, 15 December 2024

H1299

H1299 is a human non-small cell lung carcinoma (NSCLC) cell line that is widely used in cancer research. This cell line is derived from the lung tissue of a patient with non-small cell lung cancer, which is the most common type of lung cancer. H1299 cells are known for their utility in studying the molecular mechanisms of lung cancer, drug resistance, and potential therapeutic targets.

Characteristics[edit]

H1299 cells are epithelial in nature and exhibit several key characteristics that make them valuable for research:

p53 Null: H1299 cells are notable for lacking the tumor suppressor protein p53, which is a common mutation in many cancers. This makes them an important model for studying the role of p53 in cancer biology.
Growth Properties: These cells grow adherently in culture and have a doubling time of approximately 24-36 hours under optimal conditions.
Genetic Profile: H1299 cells have been extensively characterized genetically, providing a wealth of information for researchers studying gene expression and mutation effects.

Applications in Research[edit]

H1299 cells are used in a variety of research applications, including:

Cancer Biology: Researchers use H1299 cells to study the pathways involved in lung cancer progression and metastasis.
Drug Testing: These cells are used to screen potential anti-cancer drugs and to study mechanisms of drug resistance.
Gene Editing: The absence of p53 makes H1299 cells a useful model for CRISPR-Cas9 gene editing experiments to study the effects of restoring or modifying p53 function.

Culturing H1299 Cells[edit]

Culturing H1299 cells requires specific conditions to maintain their growth and viability:

Medium: H1299 cells are typically cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and antibiotics such as penicillin and streptomycin.
Temperature and Atmosphere: The cells are maintained at 37°C in a humidified atmosphere with 5% CO2.
Subculturing: Cells should be subcultured when they reach 70-80% confluence to prevent overgrowth and maintain healthy cell populations.

Research Findings[edit]

Studies using H1299 cells have contributed to significant findings in cancer research, such as:

Role of p53 in Cancer: Research has shown how the absence of p53 in H1299 cells affects cell cycle regulation and apoptosis.
Targeted Therapies: H1299 cells have been used to identify potential targets for therapy, such as specific kinases and signaling pathways involved in lung cancer.

Also see[edit]

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-'''H1299''' is a human lung carcinoma cell line that is commonly used in scientific research. This cell line is particularly useful for studying the function of the p53 gene, as it lacks this gene. The H1299 cell line is also used in studies of lung cancer, gene expression, and drug resistance.
+H1299
-== History ==
+H1299 is a human non-small cell lung carcinoma (NSCLC) cell line that is widely used in cancer research. This cell line is derived from the lung tissue of a patient with non-small cell lung cancer, which is the most common type of lung cancer. H1299 cells are known for their utility in studying the molecular mechanisms of lung cancer, drug resistance, and potential therapeutic targets.
-The H1299 cell line was established in 1980 from the lymph node metastasis of a 43-year-old Caucasian male with primary lung carcinoma. The cell line was derived by A.F. Gazdar, J.D. Minna, and colleagues at the National Cancer Institute (NCI) and the University of Texas Southwestern Medical Center.
-== Characteristics ==
+==Characteristics==
-H1299 cells are epithelial in morphology and grow in adherent culture. They are negative for the p53 gene, making them a useful model for studying the function of this gene. The cells are also resistant to many common chemotherapeutic drugs, making them a valuable tool for studying drug resistance in lung cancer.
-== Applications ==
+H1299 cells are epithelial in nature and exhibit several key characteristics that make them valuable for research:
-H1299 cells are widely used in scientific research. They are particularly useful for studying the function of the p53 gene, as they lack this gene. The cells are also used in studies of lung cancer, gene expression, and drug resistance.
-== References ==
+* '''[[p53 Null:]]''' H1299 cells are notable for lacking the tumor suppressor protein p53, which is a common mutation in many cancers. This makes them an important model for studying the role of p53 in cancer biology.
-# Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 35: 69-77, 1985.
+* '''[[Growth Properties:]]''' These cells grow adherently in culture and have a doubling time of approximately 24-36 hours under optimal conditions.
-# Minna JD, et al. Molecular biology of lung cancer: Clinical implications. Clin. Chest Med. 13: 415-429, 1992.
+* '''[[Genetic Profile:]]''' H1299 cells have been extensively characterized genetically, providing a wealth of information for researchers studying gene expression and mutation effects.
-[[Category:Cell Lines]]
+==Applications in Research==
-[[Category:Lung Cancer]]
-[[Category:Medical Research]]
-[[Category:Oncology]]
-{{stub}}
+H1299 cells are used in a variety of research applications, including:
+* '''[[Cancer Biology:]]''' Researchers use H1299 cells to study the pathways involved in lung cancer progression and metastasis.
+* '''[[Drug Testing:]]''' These cells are used to screen potential anti-cancer drugs and to study mechanisms of drug resistance.
+* '''[[Gene Editing:]]''' The absence of p53 makes H1299 cells a useful model for CRISPR-Cas9 gene editing experiments to study the effects of restoring or modifying p53 function.
+==Culturing H1299 Cells==
+Culturing H1299 cells requires specific conditions to maintain their growth and viability:
+* '''[[Medium:]]''' H1299 cells are typically cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and antibiotics such as penicillin and streptomycin.
+* '''[[Temperature and Atmosphere:]]''' The cells are maintained at 37°C in a humidified atmosphere with 5% CO2.
+* '''[[Subculturing:]]''' Cells should be subcultured when they reach 70-80% confluence to prevent overgrowth and maintain healthy cell populations.
+==Research Findings==
+Studies using H1299 cells have contributed to significant findings in cancer research, such as:
+* '''[[Role of p53 in Cancer:]]''' Research has shown how the absence of p53 in H1299 cells affects cell cycle regulation and apoptosis.
+* '''[[Targeted Therapies:]]''' H1299 cells have been used to identify potential targets for therapy, such as specific kinases and signaling pathways involved in lung cancer.
+==Also see==
+* [[Non-small cell lung carcinoma]]
+* [[p53 tumor suppressor]]
+* [[Cancer cell line]]
+* [[CRISPR-Cas9]]
+{{Lung cancer}}
+{{Cell biology}}
+[[Category:Cell lines]]
+[[Category:Cancer research]]
+[[Category:Lung cancer]]