Neutral red: Difference between revisions
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{{Short description| | |||
{{Short description|Information about the dye Neutral Red}} | |||
{{Infobox chemical | {{Infobox chemical | ||
| | | verifiedfields = changed | ||
| image = | | Watchedfields = changed | ||
| | | verifiedrevid = 477002679 | ||
| | | image = Neutral red.svg | ||
| pin = | | imagefile1 = Neutral red 3D ball.png | ||
| imagefile2 = Neutral red 3D spacefill.png | |||
| pin = C15H17ClN4 | |||
| othernames = Toluylene red, Basic red 5 | | othernames = Toluylene red, Basic red 5 | ||
}} | }} | ||
'''Neutral | '''Neutral Red''' is a [[eukaryotic]] [[vital stain]] used for [[staining]] [[lysosomes]]. It is commonly used in [[cell biology]] and [[histology]] to identify [[living cells]] and [[tissues]]. | ||
== | ==Properties== | ||
Neutral | Neutral Red is a [[weakly basic dye]] that is soluble in [[water]] and [[ethanol]]. It has a [[molecular formula]] of C15H17ClN4 and a [[molecular weight]] of 288.78 g/mol. The dye appears as a dark green powder that turns red when dissolved in water. | ||
==Applications== | ==Applications== | ||
Neutral | Neutral Red is used in various [[biological assays]] to assess [[cell viability]] and [[cytotoxicity]]. It is particularly useful in the [[Neutral Red uptake assay]], which measures the ability of cells to incorporate and bind the dye within their [[lysosomes]]. This assay is often used to evaluate the [[cytotoxic effects]] of [[chemical compounds]] and [[environmental toxins]]. | ||
==Mechanism of Action== | ==Mechanism of Action== | ||
The dye penetrates the [[cell membrane]] and accumulates in the [[lysosomes]] due to the acidic environment within these organelles. The accumulation of Neutral Red in lysosomes is a function of the [[pH gradient]] across the lysosomal membrane, which is maintained by the [[proton pump]] activity. | |||
==Safety and Handling== | ==Safety and Handling== | ||
Neutral | Neutral Red should be handled with care, as it can be harmful if ingested or inhaled. Appropriate [[personal protective equipment]] (PPE) such as [[gloves]] and [[safety goggles]] should be worn when handling the dye. | ||
== | ==See also== | ||
* [[Vital stain]] | * [[Vital stain]] | ||
* [[Cell viability assay]] | |||
* [[Lysosome]] | * [[Lysosome]] | ||
{{ | ==References== | ||
{{Reflist}} | |||
==External links== | |||
* [https://www.wikimd.com/wiki/Neutral_red Neutral Red on WikiMD] | |||
[[Category:Staining dyes]] | [[Category:Staining dyes]] | ||
[[Category: | [[Category:Histology]] | ||
[[Category:Cell biology]] | [[Category:Cell biology]] | ||
[[Category:Vital stains]] | |||
Latest revision as of 16:47, 29 December 2024
Information about the dye Neutral Red
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Neutral Red is a eukaryotic vital stain used for staining lysosomes. It is commonly used in cell biology and histology to identify living cells and tissues.
Properties[edit]
Neutral Red is a weakly basic dye that is soluble in water and ethanol. It has a molecular formula of C15H17ClN4 and a molecular weight of 288.78 g/mol. The dye appears as a dark green powder that turns red when dissolved in water.
Applications[edit]
Neutral Red is used in various biological assays to assess cell viability and cytotoxicity. It is particularly useful in the Neutral Red uptake assay, which measures the ability of cells to incorporate and bind the dye within their lysosomes. This assay is often used to evaluate the cytotoxic effects of chemical compounds and environmental toxins.
Mechanism of Action[edit]
The dye penetrates the cell membrane and accumulates in the lysosomes due to the acidic environment within these organelles. The accumulation of Neutral Red in lysosomes is a function of the pH gradient across the lysosomal membrane, which is maintained by the proton pump activity.
Safety and Handling[edit]
Neutral Red should be handled with care, as it can be harmful if ingested or inhaled. Appropriate personal protective equipment (PPE) such as gloves and safety goggles should be worn when handling the dye.
See also[edit]
References[edit]
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