Microinjection

Microinjection is a method of introducing a substance into a cell, usually to change its properties or behavior. This technique is commonly used in genetic engineering and cell biology to deliver DNA or chemicals into cells.

Overview

Microinjection is a simple mechanical process in which a glass micropipette is used to inject a liquid substance at a microscopic or borderline macroscopic level. The target is often a living cell but can also include intercellular space. This process is often used to manipulate cells and to study the effects of introducing foreign substances into cells.

History

The technique of microinjection has been developed and refined over the past century. The first reported microinjection was carried out by Julius Richard Petri in 1887, when he injected colored dyes into cells to study their properties.

Technique

Microinjection is performed under a microscope using a glass micropipette. The micropipette is filled with the substance to be injected. It is then inserted into the cell through the cell membrane. The substance is then released into the cell. The process is monitored under the microscope to ensure that the substance is correctly delivered.

Applications

Microinjection has a wide range of applications in various fields of study. In genetics, it is used to introduce new DNA into cells. In cell biology, it is used to study the functions of different parts of the cell. In medicine, it is used in gene therapy to treat genetic diseases.

See also

• Genetic engineering
• Cell biology
• Gene therapy

References

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