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'''Sequencing''' refers to the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.

==History==
The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a more automated and much higher throughput, DNA sequencing has become a common method in biological research and medical testing.

==Types of Sequencing==
There are several types of sequencing, including [[Whole Genome Sequencing]], [[Exome Sequencing]], and [[Targeted Sequencing]]. Each type has its own advantages and disadvantages, and the choice of which to use depends on the specific needs of the research or testing being done.

==Applications==
Sequencing can be used in a wide range of biological research applications, including genotyping, mutation detection, and the study of disease processes. In medicine, it can be used for prenatal testing, diagnosis of disease, and personalized medicine.

==Challenges==
Despite the many advances in sequencing technology, there are still challenges to be overcome. These include the high cost of sequencing, the difficulty of interpreting the vast amount of data generated, and ethical issues related to genetic testing.

==See Also==
* [[Genomics]]
* [[Bioinformatics]]
* [[Genetic Testing]]
* [[Personalized Medicine]]

[[Category:Genomics]]
[[Category:Bioinformatics]]
[[Category:Genetic Testing]]
[[Category:Personalized Medicine]]

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Sequencing - Revision history

Prab: CSV import

Prab: CSV import