Prab: CSV import

2025-03-03T06:21:33Z

CSV import

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			<gallery>
			File:RBC Membrane Proteins SDS-PAGE gel.jpg\|RBC Membrane Proteins SDS-PAGE gel
			File:Protein-SDS interaction.png\|Protein-SDS interaction
			File:Process of Denaturation.svg\|Process of Denaturation
			File:Electrophoresis gel combs 2.jpg\|Electrophoresis gel combs
			File:Acrylamidgel.JPG\|Acrylamide gel
			File:Disulfide reduction by DTT-2.png\|Disulfide reduction by DTT
			File:Gelelektrophoreseapparatur.jpg\|Gelelektrophorese apparatus
			File:SDSPAGE.jpg\|SDS-PAGE
			File:Coomassie3.jpg\|Coomassie
			File:Gel Blue Coomassie.jpg\|Gel Blue Coomassie
			</gallery>

Prab: CSV import

2024-02-25T17:51:26Z

CSV import

New page

'''SDS-PAGE''' or '''Sodium dodecyl sulfate polyacrylamide gel electrophoresis''' is a technique widely used in biochemistry, forensics, genetics, molecular biology and biotechnology to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature.

== Overview ==
SDS-PAGE is a method that enables the separation of molecules by size. The technique is based on the principle that a charged molecule will migrate in an electric field towards an electrode with opposite sign. The rate of migration of a molecule through a gel is inversely proportional to the logarithm of its size. Therefore, smaller molecules will migrate faster and further than larger molecules.

== Procedure ==
The procedure of SDS-PAGE can be divided into several steps:
# Sample preparation: The protein sample is mixed with a buffer containing [[SDS (Sodium dodecyl sulfate)|SDS]] and a reducing agent. The mixture is then heated to denature the proteins.
# Gel preparation: The gel is prepared by polymerizing a mixture of acrylamide and bis-acrylamide.
# Electrophoresis: The samples are loaded into the wells of the gel and an electric current is applied. The proteins migrate towards the positive electrode at a rate proportional to their size.
# Staining: After electrophoresis, the gel is stained to visualize the proteins.

== Applications ==
SDS-PAGE is used in various fields for different purposes:
# In biochemistry and molecular biology, it is used to determine the molecular weight of proteins and to analyze their relative abundance in different samples.
# In forensics, it is used to compare samples from crime scenes with samples from suspects.
# In biotechnology, it is used to monitor the purity of protein products.

== See also ==
* [[Protein electrophoresis]]
* [[Gel electrophoresis]]
* [[Polyacrylamide gel electrophoresis]]

[[Category:Biochemistry methods]]
[[Category:Molecular biology]]
[[Category:Biotechnology]]
[[Category:Forensic techniques]]

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← Older revision		Revision as of 06:21, 3 March 2025
Line 28:		Line 28:

	{{stub}}		{{stub}}
			<gallery>
			File:RBC Membrane Proteins SDS-PAGE gel.jpg\|RBC Membrane Proteins SDS-PAGE gel
			File:Protein-SDS interaction.png\|Protein-SDS interaction
			File:Process of Denaturation.svg\|Process of Denaturation
			File:Electrophoresis gel combs 2.jpg\|Electrophoresis gel combs
			File:Acrylamidgel.JPG\|Acrylamide gel
			File:Disulfide reduction by DTT-2.png\|Disulfide reduction by DTT
			File:Gelelektrophoreseapparatur.jpg\|Gelelektrophorese apparatus
			File:SDSPAGE.jpg\|SDS-PAGE
			File:Coomassie3.jpg\|Coomassie
			File:Gel Blue Coomassie.jpg\|Gel Blue Coomassie
			</gallery>

SDS-PAGE - Revision history

Prab: CSV import

Prab: CSV import