https://wikimd.org/index.php?action=history&feed=atom&title=Puromycin
Puromycin - Revision history
2026-05-13T05:20:10Z
Revision history for this page on the wiki
MediaWiki 1.44.2
https://wikimd.org/index.php?title=Puromycin&diff=6496023&oldid=prev
Prab at 00:58, 17 March 2025
2025-03-17T00:58:10Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 00:58, 17 March 2025</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">{{No image}}</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Puromycin ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Puromycin ==</div></td></tr>
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Prab
https://wikimd.org/index.php?title=Puromycin&diff=6341728&oldid=prev
Prab: CSV import
2025-02-23T21:18:56Z
<p>CSV import</p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:18, 23 February 2025</td>
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<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">File:Puromycin_skeletal.svg|Puromycin skeletal structure</ins></div></td></tr>
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Prab
https://wikimd.org/index.php?title=Puromycin&diff=6249443&oldid=prev
Prab: CSV import
2025-02-10T20:11:53Z
<p>CSV import</p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 20:11, 10 February 2025</td>
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Prab
https://wikimd.org/index.php?title=Puromycin&diff=5279365&oldid=prev
Prab: CSV import
2024-02-25T04:13:05Z
<p>CSV import</p>
<p><b>New page</b></p><div>'''Puromycin''' is an [[aminonucleoside]] [[antibiotic]], derived from the [[Streptomyces alboniger]] bacterium, that causes premature chain termination during [[translation (biology)|translation]] taking place in the [[ribosome]]. In [[molecular biology]], puromycin is used in laboratories, among other things, to study protein synthesis.<br />
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== Structure and properties ==<br />
Puromycin is structurally similar to [[aminoacyl tRNA]]. It is taken up in the A site of the ribosome during translation, causes premature chain termination by acting as an analog of the 3'-end of [[tyrosyl tRNA]]. This property leads to the incorporation of puromycin into the nascent chain and the subsequent release of the polypeptide.<br />
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== Uses ==<br />
In molecular biology, puromycin is used to study protein synthesis. By incorporating itself into the growing protein chain, the formation of long proteins is prevented, allowing the study of their synthesis. Puromycin is also used as a selective agent in laboratory cultures. It kills all cells that have not been transformed with a resistance gene (pac gene).<br />
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== Resistance ==<br />
Resistance to puromycin is conferred by the pac gene, encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain. This enzyme inactivates puromycin by transferring an acetyl group from acetyl coenzyme A to the primary amine of puromycin.<br />
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== See also ==<br />
* [[Aminonucleoside]]<br />
* [[Antibiotic]]<br />
* [[Streptomyces alboniger]]<br />
* [[Translation (biology)]]<br />
* [[Ribosome]]<br />
* [[Aminoacyl tRNA]]<br />
* [[Tyrosyl tRNA]]<br />
* [[Molecular biology]]<br />
* [[Protein synthesis]]<br />
* [[Pac gene]]<br />
* [[Puromycin N-acetyl-transferase]]<br />
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[[Category:Antibiotics]]<br />
[[Category:Aminonucleosides]]<br />
[[Category:Protein biosynthesis]]<br />
[[Category:Molecular biology]]<br />
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{{stub}}<br />
{{dictionary-stub1}}</div>
Prab