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<p>CSV import</p> <p><b>New page</b></p><div>&#039;&#039;&#039;Polyacrylamide gel&#039;&#039;&#039; is a type of [[gel]] used in [[biochemistry]], [[molecular biology]], [[genetics]], and [[clinical chemistry]] to separate biological [[macromolecules]], usually [[proteins]] or [[nucleic acids]], based on their physical properties.<br /> <br /> == Composition ==<br /> Polyacrylamide gel is composed of [[acrylamide]] and [[bisacrylamide]] monomers that are cross-linked in order to form a mesh-like network. The ratio of acrylamide to bisacrylamide can be varied to create gels with different pore sizes, allowing for the separation of molecules of different sizes.<br /> <br /> == Use in Gel Electrophoresis ==<br /> Polyacrylamide gel is most commonly used in a technique known as [[polyacrylamide gel electrophoresis]] (PAGE). In this process, a sample is loaded into a well in the gel and an electric current is applied. The molecules in the sample move through the gel at different rates depending on their size, charge, and shape, allowing them to be separated.<br /> <br /> == Variations ==<br /> There are several variations of polyacrylamide gel electrophoresis, including [[SDS-PAGE]], which uses sodium dodecyl sulfate to denature proteins and give them a negative charge, and [[two-dimensional gel electrophoresis]], which separates proteins first by their isoelectric point and then by their size.<br /> <br /> == Safety ==<br /> Polyacrylamide gel is generally safe to handle, but the acrylamide monomer is a potent neurotoxin and is potentially carcinogenic. Therefore, care must be taken when handling acrylamide powder or solutions.<br /> <br /> == See Also ==<br /> * [[Agarose gel electrophoresis]]<br /> * [[Capillary electrophoresis]]<br /> * [[Isoelectric focusing]]<br /> <br /> [[Category:Biochemistry methods]]<br /> [[Category:Molecular biology]]<br /> [[Category:Genetics]]<br /> [[Category:Clinical chemistry]]<br /> {{Biochem-stub}}<br /> {{Molbio-stub}}<br /> {{Genetics-stub}}<br /> {{Chem-stub}}</div>

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