Prab: CSV import

2025-02-23T21:23:27Z

CSV import

← Older revision		Revision as of 21:23, 23 February 2025
Line 40:		Line 40:

	{{stub}}		{{stub}}
			<gallery>
			File:Olympus-BX61-fluorescence_microscope.jpg\|Olympus BX61 fluorescence microscope
			File:FluorescenceFilters_2008-09-28.svg\|Fluorescence Filters 2008-09-28
			File:FluorescenceMicroscopeSample_HerringSpermSYBRGreen.jpg\|Fluorescence Microscope Sample Herring Sperm SYBR Green
			File:Depth_Coded_Phalloidin_Stained_Actin_Filaments_Cancer_Cell.png\|Depth Coded Phalloidin Stained Actin Filaments Cancer Cell
			File:Dividing_Cell_Fluorescence.jpg\|Dividing Cell Fluorescence
			File:FluorescentCells.jpg\|Fluorescent Cells
			File:3D_Dual_Color_Super_Resolution_Microscopy_Cremer_2010.png\|3D Dual Color Super Resolution Microscopy Cremer 2010
			File:FISH_13_21.jpg\|FISH 13 21
			File:Yeast_membrane_proteins.jpg\|Yeast membrane proteins
			</gallery>

Prab: CSV import

2024-02-23T06:51:14Z

CSV import

New page

'''Fluorescence microscope''' is a specific type of [[microscope]] that uses [[fluorescence]] and [[phosphorescence]] instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances.

== History ==

The fluorescence microscope was first developed by [[August Köhler]] in 1904. It was further improved by [[Oskar Heimstädt]], who published the first paper on fluorescence microscopy in 1911.

== Principle ==

The basic task of the fluorescence microscope is to let excitation light radiate the specimen and then sort out the much weaker emitted light to make up the image. The main components of the microscope are the [[light source]], the [[excitation filter]], the [[dichroic mirror]], and the [[emission filter]].

== Types of Fluorescence Microscopes ==

There are several types of fluorescence microscopes, including:

* '''[[Epifluorescence microscope]]''': This is the most common type of fluorescence microscope. It uses a dichroic mirror to direct the excitation light onto the specimen and then collect the fluorescence light.

* '''[[Confocal microscope]]''': This microscope uses a laser to excite a single plane of the specimen and then uses a pinhole to eliminate out-of-focus light.

* '''[[Two-photon excitation microscope]]''': This microscope uses two photons of lower energy to excite the specimen. This allows for deeper penetration into the specimen and reduces photobleaching.

* '''[[Super-resolution microscope]]''': This microscope surpasses the diffraction limit of light to provide higher resolution images.

== Applications ==

Fluorescence microscopes are used in various fields, including [[biology]], [[medicine]], and [[material science]]. They are particularly useful in studying cellular structures and processes, protein interactions, and understanding the internal structure of materials.

== See Also ==

* [[Microscopy]]
* [[Optical microscope]]
* [[Electron microscope]]

== References ==

<references />

[[Category:Microscopy]]
[[Category:Scientific techniques]]
[[Category:Fluorescence techniques]]

{{stub}}

← Older revision		Revision as of 21:23, 23 February 2025
Line 40:		Line 40:

	{{stub}}		{{stub}}
			<gallery>
			File:Olympus-BX61-fluorescence_microscope.jpg\|Olympus BX61 fluorescence microscope
			File:FluorescenceFilters_2008-09-28.svg\|Fluorescence Filters 2008-09-28
			File:FluorescenceMicroscopeSample_HerringSpermSYBRGreen.jpg\|Fluorescence Microscope Sample Herring Sperm SYBR Green
			File:Depth_Coded_Phalloidin_Stained_Actin_Filaments_Cancer_Cell.png\|Depth Coded Phalloidin Stained Actin Filaments Cancer Cell
			File:Dividing_Cell_Fluorescence.jpg\|Dividing Cell Fluorescence
			File:FluorescentCells.jpg\|Fluorescent Cells
			File:3D_Dual_Color_Super_Resolution_Microscopy_Cremer_2010.png\|3D Dual Color Super Resolution Microscopy Cremer 2010
			File:FISH_13_21.jpg\|FISH 13 21
			File:Yeast_membrane_proteins.jpg\|Yeast membrane proteins
			</gallery>

Fluorescence microscope - Revision history

Prab: CSV import

Prab: CSV import