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← Older revision		Revision as of 11:25, 18 February 2025
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	[[Category:Epigenetics]]		[[Category:Epigenetics]]
	[[Category:Molecular biology]]		[[Category:Molecular biology]]
			<gallery>
			File:CpG_vs_C-G_bp.svg\|CpG vs C-G base pair
			File:APRT-CpG.svg\|APRT CpG
			File:APRT-GpC.svg\|APRT GpC
			File:Cytosine_becomes_thymine.png\|Cytosine becomes thymine
			File:Cpg_island_evolution.svg\|CpG island evolution
			File:Initiation_of_DNA_demethylation_at_a_CpG_site.svg\|Initiation of DNA demethylation at a CpG site
			File:Demethylation_of_5-methylcytosine.svg\|Demethylation of 5-methylcytosine
			File:Genome_Expansion.png\|Genome Expansion
			</gallery>

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{{Short description|Detailed article on CpG sites in genetics}}
{{Use dmy dates|date=October 2023}}
{{Genetics sidebar}}

A '''CpG site''' or '''CpG dinucleotide''' is a region of DNA where a cytosine nucleotide is followed by a guanine nucleotide in the linear sequence of bases along its 5' → 3' direction. The "p" in CpG refers to the phosphodiester bond between the cytosine and the guanine. CpG sites are of particular interest in the field of [[epigenetics]] because they are often involved in the regulation of gene expression.

==Structure and Function==
CpG sites are characterized by the presence of a cytosine base followed by a guanine base, linked by a phosphate group. These sites are often found in clusters known as [[CpG islands]], which are regions with a high frequency of CpG sites. CpG islands are typically located near or within the [[promoter]] regions of genes and are often associated with [[housekeeping genes]].

===Methylation===
One of the key features of CpG sites is their ability to undergo [[DNA methylation]], a process where a methyl group is added to the cytosine base. Methylation of CpG sites is a crucial epigenetic modification that can influence gene expression. When CpG sites in a promoter region are methylated, the associated gene is often silenced. This mechanism is important for processes such as [[X-chromosome inactivation]], [[genomic imprinting]], and the suppression of [[transposable elements]].

===Role in Disease===
Aberrant methylation of CpG sites is implicated in various diseases, including [[cancer]]. In many cancers, CpG islands in the promoter regions of tumor suppressor genes become hypermethylated, leading to gene silencing and contributing to tumorigenesis. Conversely, global hypomethylation can lead to genomic instability and activation of oncogenes.

==CpG Islands==
[[CpG islands]] are regions of the genome that have a high frequency of CpG sites. They are typically 300 to 3,000 base pairs in length and have a GC content greater than 50%. CpG islands are often found at the 5' end of genes, particularly in the promoter regions, and are usually unmethylated in normal cells, allowing for active transcription of the associated genes.

==Evolutionary Considerations==
CpG sites are underrepresented in the vertebrate genome due to the tendency of methylated cytosines to undergo spontaneous deamination to thymine, leading to a mutation. This process results in a lower frequency of CpG sites over evolutionary time. However, CpG islands are conserved due to their functional importance in gene regulation.

==Research Techniques==
Several techniques are used to study CpG methylation, including [[bisulfite sequencing]], which allows for the detection of methylated cytosines by converting unmethylated cytosines to uracil, while leaving methylated cytosines unchanged. Other methods include [[methylation-specific PCR]] and [[methylated DNA immunoprecipitation]].

==Also see==
* [[DNA methylation]]
* [[Epigenetics]]
* [[Gene expression]]
* [[CpG island]]
* [[Genomic imprinting]]

[[Category:Genetics]]
[[Category:Epigenetics]]
[[Category:Molecular biology]]

CpG site - Revision history

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