Acridine yellow - Revision history

Prab at 03:14, 26 February 2025

2025-02-26T03:14:12Z

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 {{Short description|Acridine dye used as a fluorescent stain in biological research}}
 '''Acridine yellow''' is a fluorescent dye belonging to the [[acridine]] family of compounds. It is primarily used as a [[biological stain]] in various [[microscopy]] techniques and [[fluorescence microscopy]] applications. Acridine yellow is known for its ability to bind to [[nucleic acids]], making it useful in the study of [[DNA]] and [[RNA]] within cells.
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 ==Safety and Handling==
 As with many chemical dyes, acridine yellow should be handled with care. It is important to use appropriate [[personal protective equipment]] (PPE) such as gloves and goggles when working with this compound. Additionally, it should be stored in a cool, dry place away from direct sunlight to prevent degradation.
 ==Gallery==
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 </gallery>
 [[Category:Fluorescent dyes]]
 [[Category:Acridines]]
 [[Category:Biological stains]]
-== Acridine yellow ==
+{{Chemical compound}}

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2025-02-18T00:48:02Z

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← Older revision		Revision as of 00:48, 18 February 2025
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	[[Category:Biological stains]]		[[Category:Biological stains]]
	== Acridine yellow ==		== Acridine yellow ==
			<gallery>
			File:Acridine_yellow.png\|Acridine yellow
			File:Acridine-yellow-3D-balls.png\|Acridine yellow 3D balls
			</gallery>
			== Acridine_yellow ==
	<gallery>		<gallery>
	File:Acridine_yellow.png\|Acridine yellow		File:Acridine_yellow.png\|Acridine yellow
	File:Acridine-yellow-3D-balls.png\|Acridine yellow 3D balls		File:Acridine-yellow-3D-balls.png\|Acridine yellow 3D balls
	</gallery>		</gallery>

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2025-02-17T01:57:55Z

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← Older revision		Revision as of 01:57, 17 February 2025
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	[[Category:Acridines]]		[[Category:Acridines]]
	[[Category:Biological stains]]		[[Category:Biological stains]]
			== Acridine yellow ==
			<gallery>
			File:Acridine_yellow.png\|Acridine yellow
			File:Acridine-yellow-3D-balls.png\|Acridine yellow 3D balls
			</gallery>

Prab: CSV import

2025-02-11T21:20:11Z

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New page

{{Short description|Acridine dye used as a fluorescent stain in biological research}}
{{Chemical compound}}

'''Acridine yellow''' is a fluorescent dye belonging to the [[acridine]] family of compounds. It is primarily used as a [[biological stain]] in various [[microscopy]] techniques and [[fluorescence microscopy]] applications. Acridine yellow is known for its ability to bind to [[nucleic acids]], making it useful in the study of [[DNA]] and [[RNA]] within cells.

==Chemical Properties==
Acridine yellow is a [[heterocyclic compound]] with the chemical formula C15H15N3. It is characterized by its bright yellow color and its ability to fluoresce under [[ultraviolet light]]. The compound is soluble in water and various organic solvents, which makes it versatile for use in different experimental conditions.

==Applications==
Acridine yellow is widely used in the field of [[biochemistry]] and [[molecular biology]]. Its primary application is as a fluorescent stain for [[nucleic acid]]s. When bound to DNA or RNA, acridine yellow emits a bright fluorescence, allowing researchers to visualize and study the structure and function of genetic material within cells.

===Microscopy===
In [[fluorescence microscopy]], acridine yellow is used to stain [[cellular structures]] such as the [[nucleus]] and [[cytoplasm]]. It is particularly useful in distinguishing between [[live and dead cells]] in a sample, as it can penetrate cell membranes and bind to nucleic acids.

===Flow Cytometry===
Acridine yellow is also employed in [[flow cytometry]] to analyze the [[cell cycle]] and [[apoptosis]]. Its ability to bind to DNA allows for the quantification of DNA content in individual cells, providing insights into cell proliferation and death.

==Safety and Handling==
As with many chemical dyes, acridine yellow should be handled with care. It is important to use appropriate [[personal protective equipment]] (PPE) such as gloves and goggles when working with this compound. Additionally, it should be stored in a cool, dry place away from direct sunlight to prevent degradation.

==Related pages==
* [[Acridine orange]]
* [[Fluorescent dye]]
* [[Microscopy]]
* [[Nucleic acid staining]]

==Gallery==
<gallery>
File:Acridine_yellow.png|Acridine yellow structure
File:Acridine-yellow-3D-balls.png|3D model of acridine yellow
</gallery>

[[Category:Fluorescent dyes]]
[[Category:Acridines]]
[[Category:Biological stains]]