Targeted mutagenesis
Targeted Mutagenesis
Targeted mutagenesis (pronunciation: tar-get-ed myu-ta-je-ne-sis) is a molecular biology technique used to introduce specific changes into the DNA sequence of a gene. The term is derived from the Greek words "muta" meaning change, and "genesis" meaning origin.
Overview
In molecular biology, targeted mutagenesis is a process that introduces specific mutations into the genes of organisms. This technique is often used in research to understand the function of specific genes, or to create organisms with desired traits. The process involves the use of various techniques such as CRISPR-Cas9, Zinc finger nucleases (ZFNs), and Transcription activator-like effector nucleases (TALENs).
Techniques
CRISPR-Cas9
CRISPR-Cas9 is a revolutionary gene-editing system that allows for precise, directed changes to genomic DNA. It has been applied in the field of targeted mutagenesis to create specific gene modifications in a variety of organisms.
Zinc Finger Nucleases
Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. ZFNs can be used to introduce targeted mutations into a genome.
Transcription Activator-Like Effector Nucleases
Transcription activator-like effector nucleases (TALENs) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are used in genetic engineering and gene therapy to introduce site-specific genetic changes.
Applications
Targeted mutagenesis has a wide range of applications in various fields such as genetics, biotechnology, and medicine. It is used in the study of gene function, the development of genetically modified organisms, and the treatment of genetic diseases.
See Also
External links
- Medical encyclopedia article on Targeted mutagenesis
- Wikipedia's article - Targeted mutagenesis
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