Safranin
Safranin
Safranin (pronunciation: /ˈsafrənɪn/) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring all cell nuclei red.
Etymology
The term "Safranin" is derived from the French word "safran" which means "saffron" due to its bright red-orange color, similar to the color of saffron.
Usage
Safranin is most commonly used in the Gram stain procedure, where it functions as a counterstain to the crystal violet stain. It is also used in the endospore staining technique, where it stains vegetative cells red or pink while endospores remain green.
Preparation
Safranin is typically prepared as a 0.5% to 1% aqueous or alcohol solution. The staining process involves applying the safranin to the sample, allowing it to sit for a specified amount of time, and then rinsing off the excess stain.
Related Terms
- Gram stain: A staining technique used to classify bacteria.
- Counterstain: A stain with colour contrasting to the principal stain, making the stained structure easily visible.
- Endospore: A dormant, tough, and non-reproductive structure produced by certain bacteria.
See Also
External links
- Medical encyclopedia article on Safranin
- Wikipedia's article - Safranin
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