Replica plating

Replica Plating

Replica plating (/rɪˈplɪkə ˈpleɪtɪŋ/) is a microbiological technique that is used to replicate microorganism colonies from one agar plate to another. The technique was developed by Esther Lederberg and Joshua Lederberg in 1952.

Etymology

The term "replica plating" is derived from the English words "replica", meaning a copy, and "plating", which in microbiology refers to the process of transferring microorganisms to a growth medium.

Procedure

The process of replica plating involves the following steps:

1. A velvet pad is pressed onto the surface of an agar plate containing colonies of microorganisms.
2. The velvet pad, now carrying a replica of the colonies, is then pressed onto the surface of a second agar plate.
3. The second agar plate is incubated, allowing the microorganisms to grow and form colonies identical to those on the original plate.

Applications

Replica plating is primarily used in genetic research to isolate and study mutant strains of microorganisms. It is also used in antibiotic resistance studies to compare the growth of antibiotic-resistant and non-resistant strains of bacteria.

Related Terms

• Microorganism
• Agar plate
• Esther Lederberg
• Joshua Lederberg
• Velvet pad
• Genetic research
• Mutant
• Antibiotic resistance

See Also

• Plating (microbiology)
• Microbiology
• Genetics
• Antibiotic resistance

External links

• Medical encyclopedia article on Replica plating
• Wikipedia's article - Replica plating

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