Pepscan
Pepscan
Pepscan (pronounced: pep-scan) is a technique used in molecular biology to identify and study protein-protein interactions. The term is derived from the words 'peptide' and 'scan', indicating the method's focus on scanning peptide sequences.
Etymology
The term 'Pepscan' is a portmanteau of the words 'peptide', which refers to a compound consisting of two or more amino acids linked in a chain, and 'scan', which in this context refers to the systematic examination of protein sequences.
Method
The Pepscan method involves synthesizing overlapping peptides from a protein sequence and then testing these peptides for activity. This allows researchers to identify the specific regions of the protein that are involved in binding interactions. The technique is particularly useful in the study of antibody-antigen interactions, enzyme-substrate interactions, and protein-protein interactions.
Related Terms
- Peptide: A compound consisting of two or more amino acids linked in a chain.
- Protein: A large molecule composed of one or more chains of amino acids in a specific order.
- Amino Acid: Organic compounds that combine to form proteins.
- Antibody: A blood protein produced in response to and counteracting a specific antigen.
- Antigen: A toxin or other foreign substance that induces an immune response in the body.
- Enzyme: A substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction.
- Substrate: A substance on which an enzyme acts.
External links
- Medical encyclopedia article on Pepscan
- Wikipedia's article - Pepscan
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