Liquid chromatography
Liquid Chromatography
Liquid chromatography (pronunciation: /ˈlɪk.wɪd ˌkroʊ.məˈtɒɡ.rə.fi/) is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material.
Etymology
The term "liquid chromatography" is derived from the Greek words chroma meaning color and graphein meaning to write. The technique was first described in the early 20th century for the separation of colored compounds.
Types of Liquid Chromatography
There are several types of liquid chromatography, each with its own specific uses and advantages. These include:
- High-performance liquid chromatography (HPLC)
- Ultra high performance liquid chromatography (UHPLC)
- Size-exclusion chromatography (SEC)
- Ion-exchange chromatography (IEC)
- Affinity chromatography (AC)
Applications
Liquid chromatography is used in various fields such as biochemistry, pharmaceutical industry, food and beverage industry, and environmental testing. It is particularly useful in the detection and identification of organic compounds.
Related Terms
- Chromatography
- Gas chromatography
- Thin-layer chromatography
- Column chromatography
- Paper chromatography
External links
- Medical encyclopedia article on Liquid chromatography
- Wikipedia's article - Liquid chromatography
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